Bacteriocin: Colicin-V (Microcin-V)

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Bacteriocin: Colicin-V (Microcin-V)

Accession: BAC120

Classification
Class: Unclassified
Genetics
Gene: cvaC
Producer and target organisms

Producer Organism: Escherichia coli [Gram-negative]

Taxonomy: BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Target organismsactive against Escherichia coli (also closely related bacteria) - Enterobacteriaceae

Description
Mode of action:
Colicin V kills sensitive cells by disrupting the membrane potential.
Colicins are polypeptide toxins. export system.
Uniprot and PDB links

UniProt Entry: P22522

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=91065315;PubMed=2249654;
Gilson L., Mahanty H.K., Kolter R.
"Genetic analysis of an MDR-like export system: the secretion of colicin V.", EMBO J. 9:3875-3884(1990).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Various strains
Pinou T., Riley M.A.
"", Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=98416173;PubMed=9743528;DOI=10.1084/jem.188.6.1091
Otto B.R., van Dooren S.J.M., Nuijens J.H., Luirink J., Oudega B.
"Characterization of a hemoglobin protease secreted by the pathogenic Escherichia coli strain EB1.", J. Exp. Med. 188:1091-1103(1998).
PROTEIN SEQUENCE OF 16-62, AND DISULFIDE BOND.
MEDLINE=95039899;PubMed=7952189;
Havarstein L.S., Holo H., Nes I.F.
"The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by Gram-positive bacteria.", Microbiology 140:2383-2389(1994).
PROTEIN SEQUENCE OF 16-28, AND SUBCELLULAR LOCATION.
PubMed=8204625;
Fath M.J., Zhang L.H., Rush J., Kolter R.
"Purification and characterization of colicin V from Escherichia coli culture supernatants.", Biochemistry 33:6911-6917(1994).
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Gene structure
genome of Colicin-V  (Microcin-V)

Gene idNameDescriptionLocation
BACGene570is30IS30 transposase highly similar to X00792complement(1..402)
BACGene571is911IS911 hypothetical insertion element IS911, similar to E. coli K12 hypothetical IS911 protein (U70214)complement(359..727)
BACGene572is911hypothetical protein orf1823..1245
BACGene573hbphaemoglobin protease highly similar to L274231368..5501
BACGene574hbphypothetical protein orf2 highly similar to X636665610..6059
BACGene575hbphypothetical protein orf3 similar to AE000077complement(6483..7742)
BACGene576hbphypothetical protein orf4 similar to AE000077complement(7940..8287)
BACGene577hbphypothetical protein orf5 similar to Z73101complement(8923..9723)
BACGene578hbphypothetical protein orf6 similar to Y14081complement(9857..10738)
BACGene579cvicolicin V immunity protein related sequence X5752510929..11165
BACGene580cvaCcolicin V related sequence X5752511143..11454
BACGene581cvaBcolicin V exporter protein related sequence X57525complement(11624..12606)
is30is911is911hbphbphbphbphbphbpcvicvaCcvaB
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 ASGRDIAMAI GTLSGQFVAG GIGAAAGGVA GGAIYDYAST HKPNPAMSPS GLGGTIKQKP EGIPSEAWNY AAGRLCNWSP
 NNLSDVCL

Wheel representation
wheel representation of Colicin-V  (Microcin-V)
Protein sequence annotations
Features of Colicin-V  (Microcin-V)


FeaturePosition(s)LengthDescription
CHAIN1↔8888
Colicin-V.
Feature identifier = PRO_0000005676.
DISULFID76↔8712
Composition
Hydrophobicity

Composition
Formula C381 H594 N108 O120 S4
Absent amino acids
Common amino acids GA
Mass (Da) 8755.32
Net charge +1
Isoelectric point 7.25
Basic residues 6
Acidic residues 5
Hydrophobic residues 31
Polar residues 36
Aliphatic residues 14
Tiny residues 37
Boman Index -49.96
Hydropathy Index 0
Aliphatic Index 74.55
Instability Index 48.5 (unstable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 15595 M-1 cm-1
Absorbance 280nm 179.25

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