Bacteriocin: Microcin E492
Accession: BAC119
Classification
Class: Unclassified
Genetics
Gene: mceA
Producer and target organisms
Producer Organism: Klebsiella pneumoniae [Gram-negative]
Taxonomy: Bacteria
ProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
Target organismsenterobacteria
Description
Mode of action:
Channel-forming bacteriocin. Forms cation-selective channels.
Post-translational modification:
The C-terminal Ser is modified by attachment to a siderophore similar to enterobactin, which can bind one atom of iron. The modification consists of an ester linkage of the serine carboxyl to O6 of a glucose which is linked by a C-glycosidic bond to the 5'-benzoyl of a linear triester of N-(2,3-dihydroxybenzoyl)serine. RANGE=20-103; NOTE=Ref.2. 103 unmodified form (Ref.3). 103 modified form (Ref.3).
Channel-forming bacteriocin. Forms cation-selective channels.
Post-translational modification:
The C-terminal Ser is modified by attachment to a siderophore similar to enterobactin, which can bind one atom of iron. The modification consists of an ester linkage of the serine carboxyl to O6 of a glucose which is linked by a C-glycosidic bond to the 5'-benzoyl of a linear triester of N-(2,3-dihydroxybenzoyl)serine. RANGE=20-103; NOTE=Ref.2. 103 unmodified form (Ref.3). 103 modified form (Ref.3).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION, STRAIN=RYC492
MEDLINE=99084959;PubMed=9864332;
Lagos R., Villanueva J.E., Monasterio O.
"Identification and properties of the genes encoding microcin E492 and its immunity protein.", J. Bacteriol. 181:212-217(1999).
MEDLINE=99084959;PubMed=9864332;
Lagos R., Villanueva J.E., Monasterio O.
"Identification and properties of the genes encoding microcin E492 and its immunity protein.", J. Bacteriol. 181:212-217(1999).
[Ref. 2]
PROTEIN SEQUENCE OF 20-103, AND MASS SPECTROMETRY.
Pons A.M., Vignon D., Cottenceau G.
"", Submitted (FEB-2001) to Swiss-Prot.
Pons A.M., Vignon D., Cottenceau G.
"", Submitted (FEB-2001) to Swiss-Prot.
PARTIAL PROTEIN SEQUENCE, AMINO-ACID COMPOSITION, SIDEROPHORE BINDING SITE, AND MASS SPECTROMETRY, STRAIN=RYC492
PubMed=15102848;DOI=10.1074/jbc.M400228200
Thomas X., Destoumieux-Garzon D., Peduzzi J., Afonso C., Blond A., Birlirakis N., Goulard C., Dubost L., Thai R., Tabet J.-C., Rebuffat S.
"Siderophore peptide, a new type of post-translationally modified antibacterial peptide with potent activity.", J. Biol. Chem. 279:28233-28242(2004).
PubMed=15102848;DOI=10.1074/jbc.M400228200
Thomas X., Destoumieux-Garzon D., Peduzzi J., Afonso C., Blond A., Birlirakis N., Goulard C., Dubost L., Thai R., Tabet J.-C., Rebuffat S.
"Siderophore peptide, a new type of post-translationally modified antibacterial peptide with potent activity.", J. Biol. Chem. 279:28233-28242(2004).
CHARACTERIZATION, STRAIN=RYC492
MEDLINE=93238942;PubMed=7682973;DOI=10.1016/0014-5793(93)80096-D
Lagos R., Wilkens M., Vergara C., Cecchi X., Monasterio O.
"Microcin E492 forms ion channels in phospholipid bilayer membrane.", FEBS Lett. 321:145-148(1993).
MEDLINE=93238942;PubMed=7682973;DOI=10.1016/0014-5793(93)80096-D
Lagos R., Wilkens M., Vergara C., Cecchi X., Monasterio O.
"Microcin E492 forms ion channels in phospholipid bilayer membrane.", FEBS Lett. 321:145-148(1993).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene554 | mceA | microcin E492 precursor channel-forming bacteriocin MceA; overlapping with mceB | complement(255..554) |
| BACGene555 | mceB | microcin E492 immunity protein MceB; overlapping with mceA | complement(541..828) |
| BACGene556 | mceC | MceC required for microcin E492 modification enterochelin glycosyl transferase; similar to IroB | 1306..2418 |
| BACGene557 | mceD | MceD apo and ferric salmochelin esterase similar to IroD | complement(2597..3841) |
| BACGene558 | mceD | MceS2 similar to MchS2, microcin I47 precursor | complement(3918..4085) |
| BACGene559 | mceD | MceS3 similar to MchS3, microcin I47 immunity protein | complement(4315..4521) |
| BACGene560 | mceD | MceM | complement(4683..4940) |
| BACGene561 | mceD | MceL putative bacteriocin N- and C-termini similar to the microcin E492, I47, H47 and M precursors MceA,MchS2, MchB and McmA | complement(4962..5231) |
| BACGene562 | mceE | MceE C-terminus similar to MchS4, which promotes enterochelin production | complement(5371..5715) |
| BACGene563 | mceE | MceX similar to MshX | 5936..6043 |
| BACGene564 | mceJ | MceJ required for microcin E492 modification with salmochelin | 6182..7756 |
| BACGene565 | mceI | MceI required for microcin E492 modification with salmochelin similar to toxin-activating protein C, leukotoxin C | 7769..8260 |
| BACGene566 | mceH | MceH second component of microcin E492 exporter membrane-fusion protein; similar to CvaA, the second component of colicin Vexporter | 8419..9660 |
| BACGene567 | mceG | MceG ABC protein of microcin E492 exporter microcin leader peptide processing similar to CvaB, an ABC exporter of colicin V | 9653..11749 |
| BACGene568 | mceG | MceK putative fusion protein; N-terminus similar to McmI, microcin M immunityprotein; C-terminus similar to McmA, microcin M precursor | 11793..12203 |
| BACGene569 | mceF | MceF similar to McmM of CAAX amino terminal protease family | complement(12400..13092) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | GETDPNTQLL NDLGNNMAWG AALGAPGGLG SAALGAAGGA LQTVGQGLID HGPVNVPIPV LIGPSWNGSG SGYNSATSSS GSGS |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔84 | 84 | Microcin E492. Feature identifier = PRO_0000005677. |
| Modified residue | 84 | Serine microcin E492 siderophore ester. |
Composition
Hydrophobicity
Composition
| Formula | C338
H530
N98
O118
S1 |
| Absent amino acids | CFKR |
| Common amino acids | G |
| Mass (Da) | 7906.02 |
| Net charge | -3 |
| Isoelectric point | 3.7 |
| Basic residues | 1 |
| Acidic residues | 4 |
| Hydrophobic residues | 28 |
| Polar residues | 41 |
| Aliphatic residues | 16 |
| Tiny residues | 39 |
| Boman Index | -26.98 |
| Hydropathy Index | 0.01 |
| Aliphatic Index | 81.43 |
| Instability Index | 21.21 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 12490 M-1 cm-1 |
| Absorbance 280nm | 150.48 |
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