Bacteriocin: Enterocin SE-K4

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Bacteriocin: Enterocin SE-K4

Accession: BAC118

Classification
Class: Class IIa
Genetics
Gene: orf6
Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsUnavailable data

NUCLEOTIDE SEQUENCE, STRAIN=K-4
Doi K., Eguchi T., Iwatake A., Shima J., Ohmomo S., Ogata S.
"", Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
PROTEIN SEQUENCE, STRAIN=K-4
PubMed=11302155;
Eguchi T, Kaminaka K, Shima J, Kawamoto S, Mori K, Choi SH, Doi K, Ohmomo S, Ogata S.
"Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4.", Biosci Biotechnol Biochem. 2001, 65(2):247-53.
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Gene structure
genome of Enterocin SE-K4

Gene idNameDescriptionLocation
BACGene544orf1hypothetical protein similar to ORF5 of plasmid pYI7171..206
BACGene545orf2hypothetical protein similar to ORF6 of plasmid pYI717 similar to uvrC of plasmid pAD1208..414
BACGene546orf3hypothetical proteincomplement(654..827)
BACGene547orf4hypothetical protein similar to ORF11 of plasmid pYI717750..992
BACGene548orf5hypothetical protein similar to ORF11 of plasmid pYI717 similar to hypothetical protein ywaBcomplement(912..1160)
BACGene549orf6enterocin SE-K41414..1644
BACGene550orf7hypothetical protein enterocin precursorcomplement(2013..2252)
BACGene551orf8hypothetical protein enterocin SE-K4 homologue enterocin precursor2044..2262
BACGene552orf9enterocin immunity protein similar to ORF3 of plasmid pYI7172275..2547
BACGene553orf10hypothetical protein3008..3305
orf1orf2orf3orf4orf5orf6orf7orf8orf9orf10
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 ATYYGNGVYC NKQKCWVDWS RARSEIIDRG VKAYVNGFTK VLGGIGGR

Wheel representation
wheel representation of Enterocin SE-K4
Composition
Hydrophobicity

Composition
Formula C239 H368 N70 O67 S2
Absent amino acids HMP
Common amino acids G
Mass (Da) 5376.9
Net charge +5
Isoelectric point 9.93
Basic residues 8
Acidic residues 3
Hydrophobic residues 15
Polar residues 21
Aliphatic residues 9
Tiny residues 13
Boman Index -81.06
Hydropathy Index -0.412
Aliphatic Index 7.02
Instability Index 29.75 (stable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 17085 M-1 cm-1
Absorbance 280nm 363.51

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