Bacteriocin: Enterocin SE-K4
Accession: BAC118
Classification
Class: Class IIa
Genetics
Gene: orf6
Producer and target organisms
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q8GR39
PDB EntryUnknown
[Ref. 1]
PROTEIN SEQUENCE, STRAIN=K-4
PubMed=11302155;
Eguchi T, Kaminaka K, Shima J, Kawamoto S, Mori K, Choi SH, Doi K, Ohmomo S, Ogata S.
"Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4.", Biosci Biotechnol Biochem. 2001, 65(2):247-53.
PubMed=11302155;
Eguchi T, Kaminaka K, Shima J, Kawamoto S, Mori K, Choi SH, Doi K, Ohmomo S, Ogata S.
"Isolation and characterization of enterocin SE-K4 produced by thermophilic enterococci, Enterococcus faecalis K-4.", Biosci Biotechnol Biochem. 2001, 65(2):247-53.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene544 | orf1 | hypothetical protein similar to ORF5 of plasmid pYI717 | 1..206 |
| BACGene545 | orf2 | hypothetical protein similar to ORF6 of plasmid pYI717 similar to uvrC of plasmid pAD1 | 208..414 |
| BACGene546 | orf3 | hypothetical protein | complement(654..827) |
| BACGene547 | orf4 | hypothetical protein similar to ORF11 of plasmid pYI717 | 750..992 |
| BACGene548 | orf5 | hypothetical protein similar to ORF11 of plasmid pYI717 similar to hypothetical protein ywaB | complement(912..1160) |
| BACGene549 | orf6 | enterocin SE-K4 | 1414..1644 |
| BACGene550 | orf7 | hypothetical protein enterocin precursor | complement(2013..2252) |
| BACGene551 | orf8 | hypothetical protein enterocin SE-K4 homologue enterocin precursor | 2044..2262 |
| BACGene552 | orf9 | enterocin immunity protein similar to ORF3 of plasmid pYI717 | 2275..2547 |
| BACGene553 | orf10 | hypothetical protein | 3008..3305 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ATYYGNGVYC NKQKCWVDWS RARSEIIDRG VKAYVNGFTK VLGGIGGR |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C239
H368
N70
O67
S2 |
| Absent amino acids | HMP |
| Common amino acids | G |
| Mass (Da) | 5376.9 |
| Net charge | +5 |
| Isoelectric point | 9.93 |
| Basic residues | 8 |
| Acidic residues | 3 |
| Hydrophobic residues | 15 |
| Polar residues | 21 |
| Aliphatic residues | 9 |
| Tiny residues | 13 |
| Boman Index | -81.06 |
| Hydropathy Index | -0.412 |
| Aliphatic Index | 7.02 |
| Instability Index | 29.75 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 17085 M-1 cm-1 |
| Absorbance 280nm | 363.51 |
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