Bacteriocin: Gassericin T (gassericin K7 B)

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Bacteriocin: Gassericin T (gassericin K7 B)

Accession: BAC117

Classification
Class: Unclassified
Genetics
Gene: gatA
Producer and target organisms

Producer Organism: Lactobacillus gasseri [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsUnavailable data

Uniprot and PDB links

UniProt Entry: Q9XDR7

PDB EntryUnknown

NUCLEOTIDE SEQUENCE.
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
NUCLEOTIDE SEQUENCE, STRAIN=SBT2055
MEDLINE=21012332;PubMed=11129595;DOI=10.1271/bbb.64.2201
Kawai Y., Saitoh B., Takahashi O., Kitazawa H., Saito T.
"Primary amino acid and DNA sequences of gassericin T, a lactacin F- family bacteriocin produced by Lactobacillus gasseri SBT2055.", Biosci. Biotechnol. Biochem. 64:2201-2208(2000).
NUCLEOTIDE SEQUENCE.
Canzek Majhenic A.
"", Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE.
Zoric M., Holo H., Nes I.F., Canzek Majhenic A., Rogelj I.
"", Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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Gene structure
genome of Gassericin T (gassericin K7 B)

Gene idNameDescriptionLocation
BACGene535gatPputative pheromone peptide156..308
BACGene536gatKputative histidine kinase311..1618
BACGene537gatRputative response regulator1615..2412
BACGene538gatTputative ABC-transporter2425..4584
BACGene539gatCputative accessory protein4595..5188
BACGene540gatZhypothetical protein5256..5357
BACGene541gatAbacteriocin5490..5717
BACGene542gatXbacteriocin5727..5924
BACGene543gatIputative immunity protein5940..6278
gatPgatKgatRgatTgatCgatZgatAgatXgatI
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 RNNWAANIGG VGGATVAGWA LGNAVCGPAC GFVGAHYVPI AWAGVTAATG GFGKIRK

Wheel representation
wheel representation of Gassericin T (gassericin K7 B)
Composition
Hydrophobicity

Composition
Formula C195 H294 N58 O60 S2
Absent amino acids DEMQS
Common amino acids GA
Mass (Da) 5542.65
Net charge +5
Isoelectric point 10.26
Basic residues 5
Acidic residues 0
Hydrophobic residues 27
Polar residues 23
Aliphatic residues 10
Tiny residues 25
Boman Index 13.36
Hydropathy Index 0.46
Aliphatic Index 21.96
Instability Index 25.91 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 18115 M-1 cm-1
Absorbance 280nm 323.48

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