Bacteriocin: Gassericin T (gassericin K7 B)
Accession: BAC117
Classification
Class: Unclassified
Genetics
Gene: gatA
Producer and target organisms
Producer Organism: Lactobacillus gasseri [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q9XDR7
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
PubMed=14504837;DOI=10.1007/s00253-003-1424-2
Majhenic A.C., Venema K., Allison G.E., Matijasic B.B., Rogelj I., Klaenhammer T.R.
"DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221.", Appl. Microbiol. Biotechnol. 63:705-714(2004).
NUCLEOTIDE SEQUENCE, STRAIN=SBT2055
MEDLINE=21012332;PubMed=11129595;DOI=10.1271/bbb.64.2201
Kawai Y., Saitoh B., Takahashi O., Kitazawa H., Saito T.
"Primary amino acid and DNA sequences of gassericin T, a lactacin F- family bacteriocin produced by Lactobacillus gasseri SBT2055.", Biosci. Biotechnol. Biochem. 64:2201-2208(2000).
MEDLINE=21012332;PubMed=11129595;DOI=10.1271/bbb.64.2201
Kawai Y., Saitoh B., Takahashi O., Kitazawa H., Saito T.
"Primary amino acid and DNA sequences of gassericin T, a lactacin F- family bacteriocin produced by Lactobacillus gasseri SBT2055.", Biosci. Biotechnol. Biochem. 64:2201-2208(2000).
[Ref. 3]
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Gene structure
Gene id | Name | Description | Location |
BACGene535 | gatP | putative pheromone peptide | 156..308 |
BACGene536 | gatK | putative histidine kinase | 311..1618 |
BACGene537 | gatR | putative response regulator | 1615..2412 |
BACGene538 | gatT | putative ABC-transporter | 2425..4584 |
BACGene539 | gatC | putative accessory protein | 4595..5188 |
BACGene540 | gatZ | hypothetical protein | 5256..5357 |
BACGene541 | gatA | bacteriocin | 5490..5717 |
BACGene542 | gatX | bacteriocin | 5727..5924 |
BACGene543 | gatI | putative immunity protein | 5940..6278 |
Protein sequence
........10 ........20 ........30 ........40 ........50 ........60 | | | | | | RNNWAANIGG VGGATVAGWA LGNAVCGPAC GFVGAHYVPI AWAGVTAATG GFGKIRK |
Wheel representation
Composition
Hydrophobicity
Composition
Formula | C195
H294
N58
O60
S2 |
Absent amino acids | DEMQS |
Common amino acids | GA |
Mass (Da) | 5542.65 |
Net charge | +5 |
Isoelectric point | 10.26 |
Basic residues | 5 |
Acidic residues | 0 |
Hydrophobic residues | 27 |
Polar residues | 23 |
Aliphatic residues | 10 |
Tiny residues | 25 |
Boman Index | 13.36 |
Hydropathy Index | 0.46 |
Aliphatic Index | 21.96 |
Instability Index | 25.91 (stable) |
Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
Extinction Coefficient | 18115 M-1 cm-1 |
Absorbance 280nm | 323.48 |
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