Bacteriocin: Plantaricin 1.25 β

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Bacteriocin: Plantaricin 1.25 β

Accession: BAC115

Classification
Class: Unclassified
Genetics
Gene: plnB
Description
Lactobacillus plantarum TMW1.25 produces two bacteriocins plantaricin 1.25a and plantaricin 1.25b.
PROTEIN SEQUENCE, STRAIN=TMW1.25
PubMed=10389253;DOI=10.1046/j.1365-2672.1999.00792.x
A. Remiger, V. G. H. Eijsink, M. A. Ehrmann, K. Sletten, I. F. Nes, R. F. Vogel.
"Purification and partial amino acid sequence of plantaricin 1.25? and 1.25?, two bacteriocins produced by Lactobacillus plantarum TMW1.25", Journal of Applied Microbiology 1999, 86 (6): 1053?1058.
NUCLEOTIDE SEQUENCE, STRAIN=TMW1.25
MEDLINE=20149857;PubMed=10684981;DOI=10.1016/S0167-4781(00)00003-8
Ehrmann M.A., Remiger A., Eijsink V.GH., Vogel R.F.
"A gene cluster encoding plantaricin 1.25 and other bacteriocin-like peptides in Lactobacillus plantarum TMW1.25.", Biochim. Biophys. Acta 1490:355-361(2000).
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Gene structure
genome of Plantaricin 1.25 β

Gene idNameDescriptionLocation
BACGene532plnBplantaricin 1.25 beta bacteriocin1948..2157
plnB
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 KKKKKKVACT WGNAATAAAS GAVXGILGGP TGALAGAIWG VSQCASNNLH GMH

Wheel representation
wheel representation of Plantaricin 1.25 β
Composition
Hydrophobicity
warning: X is not a standard amino acid. warning: X is not a standard amino acid.

Composition
Formula C220 H358 N68 O62 S3
Absent amino acids DEFRY
Common amino acids A
Mass (Da) 5172.8
Net charge +8
Isoelectric point 10.8
Basic residues 8
Acidic residues 0
Hydrophobic residues 21
Polar residues 20
Aliphatic residues 8
Tiny residues 23
Boman Index -11.33
Hydropathy Index 0.083
Aliphatic Index 21.62
Instability Index 22.94 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 11125 M-1 cm-1
Absorbance 280nm 213.94

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