Bacteriocin: Plantaricin W β

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Bacteriocin: Plantaricin W β

Accession: BAC114

Classification
Genetics
Gene: PlwA
Description
Mode of action:
Plantaricin W (Plw) is a new two-peptide bacteriocin, from Lactobacillus plantarum, which inhibits a large number of Gram-positive bacteria. The two individual peptides, Plwa (comprising 29 residues) and Plwb (comprising 32 residues), had low antimicrobial activity but acted synergistically. The data indicate that the two peptides work in a 1:1 ratio. Chemical analyses showed that both peptides are lantibiotics, but two unmodified cysteines and one serine residue were present in Plwa, and Plwb contained one cysteine residue.

The Plw structural genes were sequenced and shown to encode prepeptides with sequence similarities to two other two-peptide lantibiotics, namely staphylococcin C55 and lacticin 3147.

The conserved residues are mainly serines, threonines and cysteines that can be involved in intramolecular thioether bond formation in the C-terminal parts of the molecules.

Post-translational modification:
Plwb is synthesized with a 29 aa leader, processed by an ABC transporter and then subjected to further proteolytic attack, resulting in the removal of the six Nterminal residues.
NUCLEOTIDE SEQUENCE.
MEDLINE=21135140;PubMed=11238971;
Holo H., Jeknic Z., Daeschel M., Stevanovic S., Nes I.F.
"Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics.", Microbiology 147:643-651(2001).
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Gene structure
genome of Plantaricin W β

Gene idNameDescriptionLocation
BACGene529plantaricin W beta precursor lantibiotic116..319
BACGene530plantaricin W alpha precursor lantibiotic348..527
BACGene531ABC transporter672..2792
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 SGIPCTIGAA VAASIAVCPT TKCSKRCGKR KK

Wheel representation
wheel representation of Plantaricin W β
Composition
Hydrophobicity

Composition
Formula C134 H241 N43 O39 S4
Absent amino acids DEFHLMNQWY
Common amino acids AK
Mass (Da) 3225.43
Net charge +7
Isoelectric point 10.63
Basic residues 7
Acidic residues 0
Hydrophobic residues 10
Polar residues 13
Aliphatic residues 5
Tiny residues 11
Boman Index -35.67
Hydropathy Index 0.109
Aliphatic Index 16.26
Instability Index 35.05 (stable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 8.06

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