Bacteriocin: Plantaricin W α

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Bacteriocin: Plantaricin W α

Accession: BAC109

Classification
Genetics
Gene: PlwA
Description
Mode of action:
Plantaricin W (Plw) is a new two-peptide bacteriocin, from Lactobacillus plantarum, which inhibits a large number of Gram-positive bacteria. The two individual peptides, Plwa (comprising 29 residues) and Plwb (comprising 32 residues), had low antimicrobial activity but acted synergistically. The data indicate that the two peptides work in a 1:1 ratio. Chemical analyses showed that both peptides are lantibiotics, but two unmodified cysteines and one serine residue were present in Plwa, and Plwb contained one cysteine residue.

The Plw structural genes were sequenced and shown to encode prepeptides with sequence similarities to two other two-peptide lantibiotics, namely staphylococcin C55 and lacticin 3147.

The conserved residues are mainly serines, threonines and cysteines that can be involved in intramolecular thioether bond formation in the C-terminal parts of the molecules.
NUCLEOTIDE SEQUENCE.
MEDLINE=21135140;PubMed=11238971;
Holo H., Jeknic Z., Daeschel M., Stevanovic S., Nes I.F.
"Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics.", Microbiology 147:643-651(2001).
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Gene structure
genome of Plantaricin W α

Gene idNameDescriptionLocation
BACGene502plantaricin W beta precursor lantibiotic116..319
BACGene503plantaricin W alpha precursor lantibiotic348..527
BACGene504ABC transporter672..2792
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 KCKWWNISCD LGNNGHVCTL SHECQVSCN

Wheel representation
wheel representation of Plantaricin W α
Composition
Hydrophobicity

Composition
Formula C136 H208 N42 O43 S5
Absent amino acids AFMPRY
Common amino acids C
Mass (Da) 3223
Net charge +2
Isoelectric point 7.25
Basic residues 4
Acidic residues 2
Hydrophobic residues 7
Polar residues 15
Aliphatic residues 5
Tiny residues 5
Boman Index -45.04
Hydropathy Index -0.393
Aliphatic Index 0.64
Instability Index 71.27 (unstable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 11250 M-1 cm-1
Absorbance 280nm 401.79

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