Bacteriocin: Plantaricin W α
Accession: BAC109
Classification
Class: Lantibiotic (two-peptide)
Genetics
Gene: PlwáA
Producer and target organisms
Producer Organism: Lactobacillus plantarum [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsLactobacillus spp - Lactobacillus lactis - Oenococcus oeni - Leuconostoc mesenteroides - Pediococcus acidilactici - Pediococcus pentosaceus - Enterococcus faecalis - Listeria innocua - Listeria monocytogenes - Propionibacterium freudenreichii - Staphylococcus aureus
Description
Mode of action:
Plantaricin W (Plw) is a new two-peptide bacteriocin, from Lactobacillus plantarum, which inhibits a large number of Gram-positive bacteria. The two individual peptides, Plwa (comprising 29 residues) and Plwb (comprising 32 residues), had low antimicrobial activity but acted synergistically. The data indicate that the two peptides work in a 1:1 ratio. Chemical analyses showed that both peptides are lantibiotics, but two unmodified cysteines and one serine residue were present in Plwa, and Plwb contained one cysteine residue.
The Plw structural genes were sequenced and shown to encode prepeptides with sequence similarities to two other two-peptide lantibiotics, namely staphylococcin C55 and lacticin 3147.
The conserved residues are mainly serines, threonines and cysteines that can be involved in intramolecular thioether bond formation in the C-terminal parts of the molecules.
Plantaricin W (Plw) is a new two-peptide bacteriocin, from Lactobacillus plantarum, which inhibits a large number of Gram-positive bacteria. The two individual peptides, Plwa (comprising 29 residues) and Plwb (comprising 32 residues), had low antimicrobial activity but acted synergistically. The data indicate that the two peptides work in a 1:1 ratio. Chemical analyses showed that both peptides are lantibiotics, but two unmodified cysteines and one serine residue were present in Plwa, and Plwb contained one cysteine residue.
The Plw structural genes were sequenced and shown to encode prepeptides with sequence similarities to two other two-peptide lantibiotics, namely staphylococcin C55 and lacticin 3147.
The conserved residues are mainly serines, threonines and cysteines that can be involved in intramolecular thioether bond formation in the C-terminal parts of the molecules.
Uniprot and PDB links
UniProt Entry: Q9AF67
PDB EntryUnknown
NUCLEOTIDE SEQUENCE.
MEDLINE=21135140;PubMed=11238971;
Holo H., Jeknic Z., Daeschel M., Stevanovic S., Nes I.F.
"Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics.", Microbiology 147:643-651(2001).
MEDLINE=21135140;PubMed=11238971;
Holo H., Jeknic Z., Daeschel M., Stevanovic S., Nes I.F.
"Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics.", Microbiology 147:643-651(2001).
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Gene structure
Gene id | Name | Description | Location |
BACGene502 | plantaricin W beta precursor lantibiotic | 116..319 | |
BACGene503 | plantaricin W alpha precursor lantibiotic | 348..527 | |
BACGene504 | ABC transporter | 672..2792 |
Protein sequence
........10 ........20 ........30 | | | KCKWWNISCD LGNNGHVCTL SHECQVSCN |
Wheel representation
Composition
Hydrophobicity
Composition
Formula | C136
H208
N42
O43
S5 |
Absent amino acids | AFMPRY |
Common amino acids | C |
Mass (Da) | 3223 |
Net charge | +2 |
Isoelectric point | 7.25 |
Basic residues | 4 |
Acidic residues | 2 |
Hydrophobic residues | 7 |
Polar residues | 15 |
Aliphatic residues | 5 |
Tiny residues | 5 |
Boman Index | -45.04 |
Hydropathy Index | -0.393 |
Aliphatic Index | 0.64 |
Instability Index | 71.27 (unstable) |
Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
Extinction Coefficient | 11250 M-1 cm-1 |
Absorbance 280nm | 401.79 |
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