Bacteriocin: Enterocin CRL35 (Mundticin KS)

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PROTEIN SEQUENCE
PubMed=8695065;
Far?as ME, Far?as RN, de Ruiz Holgado AP, Sesma F.
"Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35. Lett Appl Microbiol. 1996, 22(6):417-9.",
NUCLEOTIDE SEQUENCE, STRAIN=CRL35
PubMed=15215149;DOI=10.1128/AAC.48.7.2778-2781.2004
Saavedra L., Minahk C., De Ruiz Holgado A.P., Sesma F.
"Enhancement of the Enterocin CRL35 Activity by a Synthetic Peptide Derived from the NH2-Terminal Sequence.", Antimicrob. Agents Chemother. 48:2778-2781(2004).
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Gene structure
genome of Enterocin CRL35 (Mundticin KS)

Gene idNameDescriptionLocation
BACGene499munAenterocin CRL35 bacteriocin312..488
BACGene500mun Benterocin CRL 35 ATP-dependent transporter involved in translocation and processing of enterocin CRL 35630..2654
BACGene501munCenterocin CRL 35 immunity protein immunity protein2679..2975
munAmun BmunC
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 KYYGNGVSCN KKGCSVDWGK AIGIIGNNSA ANLATGGAAG WKS

Wheel representation
wheel representation of Enterocin CRL35 (Mundticin KS)
Composition
Hydrophobicity

Composition
Formula C186 H291 N55 O58 S2
Absent amino acids EFHMPQR
Common amino acids G
Mass (Da) 4308.55
Net charge +4
Isoelectric point 9.81
Basic residues 5
Acidic residues 1
Hydrophobic residues 14
Polar residues 23
Aliphatic residues 6
Tiny residues 19
Boman Index -31.82
Hydropathy Index -0.253
Aliphatic Index 15.04
Instability Index 6.27 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 14105 M-1 cm-1
Absorbance 280nm 335.83

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