Bacteriocin: Enterocin 1071A
Accession: BAC106
Classification
Class: Unclassified
Genetics
Gene: ent1071A
Producer and target organisms
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q9L9U6
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=BFE 1071
MEDLINE=20208822;PubMed=10742203;DOI=10.1128/AEM.66.4.1298-1304.2000
Balla E., Dicks L.M., Du Toit M., Van Der Merwe M.J., Holzapfel W.H.
"Characterization and cloning of the genes encoding enterocin 1071A and enterocin 1071B, two antimicrobial peptides produced by Enterococcus faecalis BFE 1071.", Appl. Environ. Microbiol. 66:1298-1304(2000).
MEDLINE=20208822;PubMed=10742203;DOI=10.1128/AEM.66.4.1298-1304.2000
Balla E., Dicks L.M., Du Toit M., Van Der Merwe M.J., Holzapfel W.H.
"Characterization and cloning of the genes encoding enterocin 1071A and enterocin 1071B, two antimicrobial peptides produced by Enterococcus faecalis BFE 1071.", Appl. Environ. Microbiol. 66:1298-1304(2000).
NUCLEOTIDE SEQUENCE, STRAIN=BFE 1071
PubMed=15718027;DOI=10.1016/j.ijfoodmicro.2004.08.008
Balla E., Dicks L.M.
"Molecular analysis of the gene cluster involved in the production and secretion of enterocins 1071A and 1071B and of the genes responsible for the replication and transfer of plasmid pEF1071.", Int. J. Food Microbiol. 99:33-45(2005).
PubMed=15718027;DOI=10.1016/j.ijfoodmicro.2004.08.008
Balla E., Dicks L.M.
"Molecular analysis of the gene cluster involved in the production and secretion of enterocins 1071A and 1071B and of the genes responsible for the replication and transfer of plasmid pEF1071.", Int. J. Food Microbiol. 99:33-45(2005).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene488 | entI1071 | EntI1071 immunity protein to enterocins 1071A and 1071B | complement(55..432) |
| BACGene489 | ent1071B | enterocin 1071B bacteriocin | complement(455..634) |
| BACGene490 | ent1071A | enterocin 1071A bacteriocin | complement(667..840) |
| BACGene491 | abc1071 | ABC transporter enterocins 1071A and 1071B transport ATP-binding protein | 1065..3158 |
| BACGene492 | entD1071 | EntD1071 probable enterocin 1071A and 1071B secretion protein | 3177..4544 |
| BACGene493 | mobC | MobC mobilization protein | 5476..5859 |
| BACGene494 | mobA | MobA mobilization protein | 5841..6881 |
| BACGene495 | mobX | MobX mobilization protein | 6607..7281 |
| BACGene496 | repA | RepA replication protein | 7837..8820 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | ESVFSKIGNA VGPAAYWILK GLGNMSDVNQ ADRINRKKH |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔39 | 39 | enterocin EntC1 peptide. Feature identifier = PRO_5000488894.
|
Composition
Hydrophobicity
Composition
| Formula | C283
H462
N82
O80
S3 |
| Absent amino acids | CT |
| Common amino acids | GANK |
| Mass (Da) | 4285.16 |
| Net charge | +4 |
| Isoelectric point | 10.48 |
| Basic residues | 7 |
| Acidic residues | 3 |
| Hydrophobic residues | 14 |
| Polar residues | 12 |
| Aliphatic residues | 8 |
| Tiny residues | 11 |
| Boman Index | -68.01 |
| Hydropathy Index | -0.462 |
| Aliphatic Index | 82.56 |
| Instability Index | 14.06 (stable) |
| Half Life |
Mammalian : 1 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 6990 M-1 cm-1 |
| Absorbance 280nm | 183.95 |
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