Bacteriocin: lactacin-F (lafA)

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Bacteriocin: lactacin-F (lafA)

Accession: BAC105

Classification
Class: class IIB
Genetics
Gene: lafA; Synonyms=laf; OrderedLocusNames=LJ_0769
Producer and target organisms
Description
Mode of action:
Heat stable bacteriocin. peptides for activity: lafA and lafX. Associated with a 180 kDa bacteriocin complex.
Uniprot and PDB links

UniProt Entry: P24022

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11506 / VPI 11088
MEDLINE=94113718;PubMed=8285694;
Fremaux C., Ahn C., Klaenhammer T.R.
"Molecular analysis of the lactacin F operon.", Appl. Environ. Microbiol. 59:3906-3915(1993).
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], STRAIN=NCC 533
PubMed=14983040;DOI=10.1073/pnas.0307327101
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.", Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE, STRAIN=ATCC 11506 / VPI 11088
MEDLINE=91154135;PubMed=1900281;
Muriana P.M., Klaenhammer T.R.
"Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.", J. Bacteriol. 173:1779-1788(1991).
PROTEIN SEQUENCE OF 19-43, STRAIN=ATCC 11506 / VPI 11088
MEDLINE=91241762;PubMed=1903624;
Muriana P.M., Klaenhammer T.R.
"Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088.", Appl. Environ. Microbiol. 57:114-121(1991).
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Gene structure
genome of lactacin-F  (lafA)

Gene idNameDescriptionLocation
BACGene477lactacin F two-component system inducer peptide precursor661355..661507
BACGene478lactacin F two-component system histidine kinase661510..662817
BACGene479lactacin F two-component system response regulator664315..665112
BACGene480lactacin F ABC transporter permease component665125..667284
BACGene481lactacin F transporter auxillary protein667295..667888
BACGene482bacteriocin lactacin F, subunit LafA precursor668015..668242
BACGene483bacteriocin lactacin F, subunit LafX precursor668253..668441
BACGene484lactacin F immunity protein668528..668902
BACGene485lactacin F two-component system response regulator fragment670446..670853
BACGene486lactacin F ABC transporter permease component670866..673025
BACGene487lactacin F transporter auxillary protein673036..673629
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 RNNWQTNVGG AVGSAMIGAT VGGTICGPAC AVAGAHYLPI LWTAVTAATG GFGKIRK

Wheel representation
wheel representation of lactacin-F  (lafA)
Protein sequence annotations
Features of lactacin-F  (lafA)


FeaturePosition(s)LengthDescription
CHAIN1↔5757
Bacteriocin lactacin-F subunit LafA.
Feature identifier = PRO_0000002754.
CONFLICT44A -> G (in Ref. 1; no nucleotide entryand 2; AAA16636).
Composition
Hydrophobicity

Composition
Formula C248 H395 N73 O70 S3
Absent amino acids DE
Common amino acids GA
Mass (Da) 5634.49
Net charge +5
Isoelectric point 10.26
Basic residues 5
Acidic residues 0
Hydrophobic residues 24
Polar residues 24
Aliphatic residues 11
Tiny residues 22
Boman Index 0.69
Hydropathy Index 0.43
Aliphatic Index 84.04
Instability Index 23.23 (stable)
Half Life Mammalian : 1 hour
Yeast : 2 min
E. coli : 2 min
Extinction Coefficient 12615 M-1 cm-1
Absorbance 280nm 225.27

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