Bacteriocin: Plantaricin NC8 β
Accession: BAC103
Classification
Class: Unclassified
Genetics
Gene: plnc8B
Producer and target organisms
Producer Organism: Lactobacillus plantarum [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q84HW1
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=NC8
PubMed=14973042;DOI=10.1128/JB.186.5.1556-1564.2004
Maldonado A., Jimenez-Diaz R., Ruiz-Barba J.L.
"Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism.", J. Bacteriol. 186:1556-1564(2004).
PubMed=14973042;DOI=10.1128/JB.186.5.1556-1564.2004
Maldonado A., Jimenez-Diaz R., Ruiz-Barba J.L.
"Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism.", J. Bacteriol. 186:1556-1564(2004).
NUCLEOTIDE SEQUENCE, STRAIN=NC8
MEDLINE=22401440;PubMed=12514019;DOI=10.1128/AEM.69.1.383-389.2003
Maldonado A., Ruiz-Barba J.L., Jimenez-Diaz R.
"Purification and genetic characterization of plantaricin NC8, a novel coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8.", Appl. Environ. Microbiol. 69:383-389(2003).
MEDLINE=22401440;PubMed=12514019;DOI=10.1128/AEM.69.1.383-389.2003
Maldonado A., Ruiz-Barba J.L., Jimenez-Diaz R.
"Purification and genetic characterization of plantaricin NC8, a novel coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8.", Appl. Environ. Microbiol. 69:383-389(2003).
[Ref. 3]
NUCLEOTIDE SEQUENCE, STRAIN=J51
Navarro L., Saenz Y., Rojo B., Zarazaga M., Ruiz-Larrea F., Torres C.
"Genetic characterization of the pln locus in the bacteriocin-producer Lactobacillus plantarum strain J51 of wine origin.", Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
Navarro L., Saenz Y., Rojo B., Zarazaga M., Ruiz-Larrea F., Torres C.
"Genetic characterization of the pln locus in the bacteriocin-producer Lactobacillus plantarum strain J51 of wine origin.", Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene444 | brnQ1 | branched-chain amino acid transport protein | 355..1731 |
| BACGene445 | napA1 | Na(+)/H(+) antiporter | 1764..2960 |
| BACGene446 | plnc8B | plantaricin NC8 beta peptide precursor contains consensus double-glycine leader sequence; bacteriocin | 3440..3607 |
| BACGene447 | plnc8A | plantaricin NC8 alpha peptide precursor bacteriocin; contains consensus double-glycine leader sequence | 3622..3765 |
| BACGene448 | plnc8C | immunity protein | 3693..3965 |
| BACGene449 | plnc8C | putative bacteriocin ORF3-J51; contains consensus double-glycine leader sequence | 5990..6163 |
| BACGene450 | plnc8C | putative bacteriocin ORF4-J51; contains consensus double-glycine leader sequence | 6179..6358 |
| BACGene451 | plnc8C | unknown ORF5-J51; unknown function | 6464..6706 |
| BACGene452 | plnc8C | unknown ORFZ3; unknown function; similar to GenBank Accession Number ABS11215 | 6935..7198 |
| BACGene453 | plnR-like | unknown contains deletion compared to PlnR in CAD63041 | complement(7165..7278) |
| BACGene454 | plnL | PlnL immunity protein | complement(7303..7971) |
| BACGene455 | plnL | unknown Orf2; similar to Orf2 in GenBank Accession Number AAL77868 | 8392..8622 |
| BACGene456 | plnQ | plantaricin biosynthesis protein PlnQ | 8707..8895 |
| BACGene457 | plnA | plantaricin A precursor peptide, induction factor | 9232..9378 |
| BACGene458 | plnB | histidine protein kinase PlnB, sensor protein ttg start codon | 9569..10897 |
| BACGene459 | plnC | response regulator PlnC, activator gtg start codon | 10898..11641 |
| BACGene460 | plnD | response regulator PlnD, repressor ttg start codon | 11760..12503 |
| BACGene461 | plnI | immunity protein PlnI, membrane-bound protease CAAX family | complement(12808..13581) |
| BACGene462 | plnF | bacteriocin precursor peptide PlnF contains consensus double-glycine leader sequence | complement(13680..13838) |
| BACGene463 | plnE | bacteriocin precursor peptide PlnE contains consensus double-glycine leader sequence | complement(13863..14033) |
| BACGene464 | plnG | bacteriocin ABC transporter ATP-binding and permease protein PlnG | 14300..16450 |
| BACGene465 | plnH | bacteriocin ABC transporter accessory factor PlnH | 16466..17842 |
| BACGene466 | plnS | plantaricin biosynthesis protein PlnS | 17932..18231 |
| BACGene467 | plnT | integral membrane protein PlnT, membrane-bound protease CAAX family | 18077..18622 |
| BACGene468 | plnU | integral membrane protein PlnU, membrane-bound protease CAAX family | 18689..19357 |
| BACGene469 | plnV | integral membrane protein PlnV, membrane-bound protease CAAX family | 19444..20124 |
| BACGene470 | plnW | integral membrane protein PlnW, membrane-bound protease CAAX family | 20218..20667 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | MNNLNKFSTL GKSSLSQIEG GSVPTSVYTL GIKILWSAYK HRKTIEKSFN KGFYH |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔34 | 34 | plantaricin NC8 beta peptide. Feature identifier = PRO_5000063925. |
Composition
Hydrophobicity
Composition
| Formula | C283
H443
N75
O80
S1 |
| Absent amino acids | CD |
| Common amino acids | SK |
| Mass (Da) | 6227.02 |
| Net charge | +8 |
| Isoelectric point | 10.6 |
| Basic residues | 10 |
| Acidic residues | 2 |
| Hydrophobic residues | 16 |
| Polar residues | 24 |
| Aliphatic residues | 11 |
| Tiny residues | 14 |
| Boman Index | -74.22 |
| Hydropathy Index | -0.41 |
| Aliphatic Index | 76.18 |
| Instability Index | 52.5 (unstable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 9970 M-1 cm-1 |
| Absorbance 280nm | 184.63 |
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