Bacteriocin: lactococcin-A

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Bacteriocin: lactococcin-A

Accession: BAC102

Classification
Class: Unclassified
Genetics
Gene: lcnA
Producer and target organisms

Producer Organism: Lactococcus lactis subsp (Streptococcus cremoris), cremoris [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsLactococci

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=9B4; PLASMID=p9B4-6
MEDLINE=91197113;PubMed=1901707;
van Belkum M.J., Hayema B.J., Jeeninga R.E., Kok J., Venema G.
"Organization and nucleotide sequences of two lactococcal bacteriocin operons.", Appl. Environ. Microbiol. 57:492-498(1991).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 22-75, STRAIN=LMG 2130
MEDLINE=91267955;PubMed=1904860;
Holo H., Nilssen O., Nes I.F.
"Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.", J. Bacteriol. 173:3879-3887(1991).
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Gene structure
genome of lactococcin-A

Gene idNameDescriptionLocation
BACGene442probacteriocin239..466
BACGene443bacteriocin immunity481..777
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 KLTFIQSTAA GDLYYNTNTH KYVYQQTQNA FGAAANTIVN GWMGGAAGGF GLHH

Wheel representation
wheel representation of lactococcin-A
Protein sequence annotations
Features of lactococcin-A


FeaturePosition(s)LengthDescription
CHAIN1↔5454
Bacteriocin lactococcin-A.
Feature identifier = PRO_0000002769.
HELIX9↔3123Potential
Composition
Hydrophobicity

Composition
Formula C260 H380 N72 O77 S1
Absent amino acids CEPR
Common amino acids AG
Mass (Da) 5797.31
Net charge +4
Isoelectric point 8.97
Basic residues 5
Acidic residues 1
Hydrophobic residues 19
Polar residues 24
Aliphatic residues 7
Tiny residues 17
Boman Index -40.24
Hydropathy Index -0.24
Aliphatic Index 61.67
Instability Index 22.35 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 11460 M-1 cm-1
Absorbance 280nm 216.23

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