Bacteriocin: Enterocin B
Accession: BAC101
Classification
Genetics
Gene: entB
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsGram_positive: Enterococcus faecalis JCM 5803 (MIC=86.9nM) - Enterococcus faecalis OU510 (MIC=174nM) - Enterococcus faecium TUA 1344L (MIC=43.4nM) - Enterococcus hirae ATCC 10541 (MIC=5.44nM) - Lactobacillus plantarum ATCC 14917 (MIC=86.9nM) - Lactobacillus sakei ssp. sakei JCM 1157 (MIC=5.44nM) - Bacillus coagulans JCM 2257 (MIC=89000nM) - Bacillus subtilis JCM 1465 (MIC=44500nM)
Uniprot and PDB links
UniProt Entry: O34017
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=T136
MEDLINE=97388583;PubMed=9245817;
Casaus P., Nilsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A.", Microbiology 143:0-0(0).
MEDLINE=97388583;PubMed=9245817;
Casaus P., Nilsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A.", Microbiology 143:0-0(0).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=T136
Casaus P., Nielsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"", Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
Casaus P., Nielsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.
"", Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=BFE 900
MEDLINE=99240446;PubMed=10224016;
Franz C.M.A.P., Worobo R.W., Quadri L.E.N., Schillinger U., Holzapfel W.H., Vederas J.C., Stiles M.E.
"Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900.", Appl. Environ. Microbiol. 65:2170-2178(1999).
MEDLINE=99240446;PubMed=10224016;
Franz C.M.A.P., Worobo R.W., Quadri L.E.N., Schillinger U., Holzapfel W.H., Vederas J.C., Stiles M.E.
"Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900.", Appl. Environ. Microbiol. 65:2170-2178(1999).
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene419 | pepC | cysteine aminopeptidase PepC | complement(1..675) |
| BACGene420 | pepC | unknown orf17 | 354..494 |
| BACGene421 | pepC | unknown orf16 | 859..1134 |
| BACGene422 | pepC | unknown orf15 | complement(1098..1259) |
| BACGene423 | pepC | unknown orf14 | 1131..1283 |
| BACGene424 | pepC | unknown orf13 | 1765..2394 |
| BACGene425 | pepC | unknown orf12 | 2414..2596 |
| BACGene426 | pepC | unknown orf11 | complement(2508..2882) |
| BACGene427 | bglB | phospho-beta-glucosidase BglB | complement(3195..4622) |
| BACGene428 | bglS | beta-glucoside specific transport protein enzyme II-Bgl; BglS | complement(4634..6490) |
| BACGene429 | bglR | transcription antiterminator BglR | complement(6483..7328) |
| BACGene430 | bglR | unknown orf2 | complement(7677..7847) |
| BACGene431 | bglR | unknown orf1 | 7686..7832 |
| BACGene432 | bglR | unknown orf3 | complement(7822..8154) |
| BACGene433 | bglR | unknown orf4 | complement(8151..8366) |
| BACGene434 | entB | enterocin B precursor bacteriocin | 8643..8858 |
| BACGene435 | eniB | enterocin B immunity protein EniB | complement(8879..9055) |
| BACGene436 | eniB | unknown orf5 | complement(9078..9269) |
| BACGene437 | eniB | induction factor-like protein orf6 | complement(9269..9388) |
| BACGene438 | eniB | unknown orf7 | 10416..10637 |
| BACGene439 | eniB | unknown orf8 | 10917..11108 |
| BACGene440 | eniB | unknown orf9 | complement(11428..11571) |
| BACGene441 | eniB | unknown orf10 | complement(11776..11949) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 | | | | | | ENDHRMPNEL NRPNNLSKGG AKCGAAIAGG LFGIPKGPLA WAAGLANVYS KCN |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C237
H377
N73
O70
S3 |
| Absent amino acids | QT |
| Common amino acids | AGN |
| Mass (Da) | 5484.14 |
| Net charge | +4 |
| Isoelectric point | 9.43 |
| Basic residues | 7 |
| Acidic residues | 3 |
| Hydrophobic residues | 18 |
| Polar residues | 20 |
| Aliphatic residues | 8 |
| Tiny residues | 18 |
| Boman Index | -61.76 |
| Hydropathy Index | -0.38 |
| Aliphatic Index | 72.08 |
| Instability Index | 13.58 (stable) |
| Half Life |
Mammalian : 1 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 7115 M-1 cm-1 |
| Absorbance 280nm | 136.83 |
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