Bacteriocin: boticin B

Accession: BAC097

Classification

Class: ~~Unclassified~~

Genetics

Gene: btcB

Producer and target organisms

Target organismsactive against various strains of Clostridium botulinum -

related clostridia

Uniprot and PDB links

UniProt Entry: Q9KGZ4

PDB Entry~~Unknown~~

NUCLEOTIDE SEQUENCE, STRAIN=213B
MEDLINE=20551134;PubMed=11097932;DOI=10.1128/AEM.66.12.5480-5483.2000
Dineen S.S., Bradshaw M., Johnson E.A.
"Cloning, nucleotide sequence, and expression of the gene encoding the bacteriocin boticin B from Clostridium botulinum strain 213B.", Appl. Environ. Microbiol. 66:5480-5483(2000).

Gene structure

Gene id	Name	Description	Location
BACGene392	dnaE	DNA polymerase III DnaE TO08_01	complement(1..3582)
BACGene393	dnaE	putative ABC transporter TO08_02	complement(3609..5141)
BACGene394	dnaE	PmbA family protein TO08_03	complement(5359..6579)
BACGene395	dnaE	TldD family protein TO08_04	complement(6566..7750)
BACGene396	dnaE	bacteriocin boticin B TO08_05	complement(7947..8099)
BACGene397	dnaE	putative bacteriocin biosynthesis protein TO08_06	complement(8367..9665)
BACGene398	dnaE	hypothetical protein TO08_07	complement(10843..11001)
BACGene399	dnaE	putative phage integrase TO08_08	11312..11887
BACGene400	dnaE	RepB family protein TO08_09	complement(12900..13919)
BACGene401	dnaE	hypothetical protein TO08_10	15438..15659
BACGene402	dnaE	hypothetical protein TO08_11	15679..15906
BACGene403	dnaE	hypothetical protein TO08_12	complement(15967..16194)
BACGene404	dnaE	hypothetical protein TO08_13	complement(16196..16681)
BACGene405	dnaE	hypothetical protein TO08_14	complement(16678..16905)
BACGene406	dnaE	putative replication protein TO08_15	complement(17121..18515)
BACGene407	dnaE	hypothetical protein TO08_16	complement(18747..19511)
BACGene408	dnaE	hypothetical protein TO08_17	complement(20235..20882)

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60
| | | | | |
MQKPEIISAD LGLCAVNEFV ALAAIPGGAA TFAVCQMPNL DEIVSNAAYV

Wheel representation

Composition

Hydrophobicity

Composition

Formula	C₂₂₈ H₃₆₄ N₅₆ O₇₀ S₄
Absent amino acids	HRW
Common amino acids	A
Mass (Da)	5156.86
Net charge	-4
Isoelectric point	3.64
Basic residues	1
Acidic residues	5
Hydrophobic residues	25
Polar residues	12
Aliphatic residues	13
Tiny residues	15
Boman Index	9.77
Hydropathy Index	0.76
Aliphatic Index	111.4
Instability Index	24.51 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	1615 M^-1 cm^-1
Absorbance 280nm	32.96

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