Bacteriocin: boticin B

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Bacteriocin: boticin B

Accession: BAC097

Class: Unclassified
Gene: btcB
Producer and target organisms

Producer Organism: Clostridium botulinum [Gram-positive]

Taxonomy: BacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Target organismsactive against various strains of Clostridium botulinum -

related clostridia

Uniprot and PDB links

UniProt Entry: Q9KGZ4

PDB EntryUnknown

Dineen S.S., Bradshaw M., Johnson E.A.
"Cloning, nucleotide sequence, and expression of the gene encoding the bacteriocin boticin B from Clostridium botulinum strain 213B.", Appl. Environ. Microbiol. 66:5480-5483(2000).
End Note
Reference Manager
Gene structure
genome of boticin B

Gene idNameDescriptionLocation
BACGene392dnaEDNA polymerase III DnaE TO08_01complement(1..3582)
BACGene393dnaEputative ABC transporter TO08_02complement(3609..5141)
BACGene394dnaEPmbA family protein TO08_03complement(5359..6579)
BACGene395dnaETldD family protein TO08_04complement(6566..7750)
BACGene396dnaEbacteriocin boticin B TO08_05complement(7947..8099)
BACGene397dnaEputative bacteriocin biosynthesis protein TO08_06complement(8367..9665)
BACGene398dnaEhypothetical protein TO08_07complement(10843..11001)
BACGene399dnaEputative phage integrase TO08_0811312..11887
BACGene400dnaERepB family protein TO08_09complement(12900..13919)
BACGene401dnaEhypothetical protein TO08_1015438..15659
BACGene402dnaEhypothetical protein TO08_1115679..15906
BACGene403dnaEhypothetical protein TO08_12complement(15967..16194)
BACGene404dnaEhypothetical protein TO08_13complement(16196..16681)
BACGene405dnaEhypothetical protein TO08_14complement(16678..16905)
BACGene406dnaEputative replication protein TO08_15complement(17121..18515)
BACGene407dnaEhypothetical protein TO08_16complement(18747..19511)
BACGene408dnaEhypothetical protein TO08_17complement(20235..20882)
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 

Wheel representation
wheel representation of boticin B

Formula C228 H364 N56 O70 S4
Absent amino acids HRW
Common amino acids A
Mass (Da) 5156.86
Net charge -4
Isoelectric point 3.64
Basic residues 1
Acidic residues 5
Hydrophobic residues 25
Polar residues 12
Aliphatic residues 13
Tiny residues 15
Boman Index 9.77
Hydropathy Index 0.76
Aliphatic Index 111.4
Instability Index 24.51 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 1615 M-1 cm-1
Absorbance 280nm 32.96

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