Bacteriocin: Brochocin-c
Accession: BAC093
Classification
Class: Unclassified
Genetics
Gene: brcA
Producer and target organisms
Producer Organism: Brochothrix campestris [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacillales
Listeriaceae
Brochothrix
Target organismsBacillus - Brochothrix - Carnobacterium - Clostridium - Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - Pediococcus - Staphylococcus - Streptococcus
Uniprot and PDB links
UniProt Entry: O85756
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=ATCC43754
MEDLINE=99054910;PubMed=9835559;
McCormick J.K., Poon A., Sailer M., Gao Y., Roy K.L., McMullen L.M., Vederas J.C., Stiles M.E., Van Belkum M.J.
"Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.", Appl. Environ. Microbiol. 64:4757-4766(1998).
MEDLINE=99054910;PubMed=9835559;
McCormick J.K., Poon A., Sailer M., Gao Y., Roy K.L., McMullen L.M., Vederas J.C., Stiles M.E., Van Belkum M.J.
"Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.", Appl. Environ. Microbiol. 64:4757-4766(1998).
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Gene structure
Gene id | Name | Description | Location |
BACGene380 | transposase ORF1 | 1..399 | |
BACGene381 | ORF2 | 417..716 | |
BACGene382 | brcA | bacteriocin BrcA; brochocin-C; antibotulinal | 1273..1506 |
BACGene383 | brcB | bacteriocin BrcB; brochocin-C; antibotulinal | 1519..1701 |
BACGene384 | brcI | bacteriocin immunity protein BrcI | 1695..1856 |
BACGene385 | brcD | transport accessory protein BrcD | 2059..2535 |
BACGene386 | brcT | ABC translocator BrcT | 2722..4357 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | YSSKDCLKDI GKGIGAGTVA GAAGGGLAAG LGAIPGAFVG AHFGVIGG |
Wheel representation
Composition
Hydrophobicity
Composition
Formula | C191
H305
N53
O57
S1 |
Absent amino acids | EMNQRW |
Common amino acids | G |
Mass (Da) | 4306.77 |
Net charge | +2 |
Isoelectric point | 8.52 |
Basic residues | 4 |
Acidic residues | 2 |
Hydrophobic residues | 21 |
Polar residues | 20 |
Aliphatic residues | 10 |
Tiny residues | 26 |
Boman Index | 35.93 |
Hydropathy Index | 0.69 |
Aliphatic Index | 93.75 |
Instability Index | 1.54 (stable) |
Half Life |
Mammalian : 2.8 hour Yeast : 10 min E. coli : 2 min |
Extinction Coefficient | 1490 M-1 cm-1 |
Absorbance 280nm | 31.7 |
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