Bacteriocin: lactacin-F (lafX)
Accession: BAC092
Classification
Class: class IIB
Genetics
Gene: lafX; OrderedLocusNames=LJ_0769.1; ORFNames=LJ_0769b
Producer and target organisms
Producer Organism: Lactobacillus johnsonii [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsLactobacilli - Enterococcus faecalis
Description
Mode of action:
Heat stable bacteriocin. peptides for activity: lafA and lafX. Associated with a 180 kDa bacteriocin complex.
Heat stable bacteriocin. peptides for activity: lafA and lafX. Associated with a 180 kDa bacteriocin complex.
Uniprot and PDB links
UniProt Entry: Q48509
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11506 / VPI 11088
MEDLINE=94113718;PubMed=8285694;
Fremaux C., Ahn C., Klaenhammer T.R.
"Molecular analysis of the lactacin F operon.", Appl. Environ. Microbiol. 59:3906-3915(1993).
MEDLINE=94113718;PubMed=8285694;
Fremaux C., Ahn C., Klaenhammer T.R.
"Molecular analysis of the lactacin F operon.", Appl. Environ. Microbiol. 59:3906-3915(1993).
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], STRAIN=NCC 533
PubMed=14983040;DOI=10.1073/pnas.0307327101
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.", Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
PubMed=14983040;DOI=10.1073/pnas.0307327101
Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C., Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R., Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.
"The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533.", Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene369 | lactacin F two-component system inducer peptide precursor | 661355..661507 | |
| BACGene370 | lactacin F two-component system histidine kinase | 661510..662817 | |
| BACGene371 | lactacin F two-component system response regulator | 664315..665112 | |
| BACGene372 | lactacin F ABC transporter permease component | 665125..667284 | |
| BACGene373 | lactacin F transporter auxillary protein | 667295..667888 | |
| BACGene374 | bacteriocin lactacin F, subunit LafA precursor | 668015..668242 | |
| BACGene375 | bacteriocin lactacin F, subunit LafX precursor | 668253..668441 | |
| BACGene376 | lactacin F immunity protein | 668528..668902 | |
| BACGene377 | lactacin F two-component system response regulator fragment | 670446..670853 | |
| BACGene378 | lactacin F ABC transporter permease component | 670866..673025 | |
| BACGene379 | lactacin F transporter auxillary protein | 673036..673629 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | NRWGDTVLSA ASGAGTGIKA CKSFGPWGMA ICGVGGAAIG GYFGYTHN |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔48 | 48 | Bacteriocin lactacin-F subunit LafX. Feature identifier = PRO_0000002756. |
Composition
Hydrophobicity
Composition
| Formula | C210
H313
N59
O61
S3 |
| Absent amino acids | EQ |
| Common amino acids | G |
| Mass (Da) | 4755.16 |
| Net charge | +3 |
| Isoelectric point | 8.77 |
| Basic residues | 4 |
| Acidic residues | 1 |
| Hydrophobic residues | 17 |
| Polar residues | 24 |
| Aliphatic residues | 6 |
| Tiny residues | 22 |
| Boman Index | -3.63 |
| Hydropathy Index | 0.2 |
| Aliphatic Index | 59.17 |
| Instability Index | 17.79 (stable) |
| Half Life |
Mammalian : 1.4 hour Yeast : 3 min E. coli : >10 hour |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 300.11 |
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