Bacteriocin: lactococcin-B
Accession: BAC087
Classification
Class: Unclassified
Genetics
Gene: lcnB
Producer and target organisms
Producer Organism: Lactococcus lactis subsp (Streptococcus cremoris), cremoris [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Streptococcaceae
Lactococcus
Target organismsLactococci
Description
Mode of action:
Kills Lactococci by dissipating the membrane potential of the cells.
Kills Lactococci by dissipating the membrane potential of the cells.
Uniprot and PDB links
UniProt Entry: P35518
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=9B4
MEDLINE=92304065;PubMed=1610182;
van Belkum M.J., Kok J., Venema G.
"Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.", Appl. Environ. Microbiol. 58:572-577(1992).
MEDLINE=92304065;PubMed=1610182;
van Belkum M.J., Kok J., Venema G.
"Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.", Appl. Environ. Microbiol. 58:572-577(1992).
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Gene structure
Gene id | Name | Description | Location |
BACGene316 | lciA | lactococcin A immunity; This sequence comes from Fig. 2 | 1..75 |
BACGene317 | lcnB | bacteriocin lactococcin B This sequence comes from Fig. 2. | 350..556 |
BACGene318 | lciB | lactococcin B immunity; This sequence comes from Fig. 2 | 583..858 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | SLQYVMSAGP YTWYKDTRTG KTICKQTIDT ASYTFGVMAE GWGKTFH |
Wheel representation
Protein sequence annotations
Feature | Position(s) | Length | Description |
CHAIN | 1↔47 | 47 | Bacteriocin lactococcin-B. Feature identifier = PRO_0000002771. |
MUTAGEN | 24 | C->A,D,E,F,G,I,L,M,N,P,Q,S,T,V,Y,W: Noloss of activity. | |
MUTAGEN | 24 | C->R,K: Loss of activity. |
Composition
Hydrophobicity
Composition
Formula | C241
H358
N60
O71
S3 |
Absent amino acids | N |
Common amino acids | T |
Mass (Da) | 5346.8 |
Net charge | +3 |
Isoelectric point | 9.1 |
Basic residues | 6 |
Acidic residues | 3 |
Hydrophobic residues | 12 |
Polar residues | 21 |
Aliphatic residues | 5 |
Tiny residues | 11 |
Boman Index | -58.86 |
Hydropathy Index | -0.44 |
Aliphatic Index | 43.62 |
Instability Index | 19.18 (stable) |
Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 16960 M-1 cm-1 |
Absorbance 280nm | 368.7 |
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