Bacteriocin: pediocin PA-1 (Pediocin ACH)

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Bacteriocin: pediocin PA-1 (Pediocin ACH)

Accession: BAC083

Classification

Class: class IIA/YGNGV

Genetics

Gene: pedA; Synonyms=pap, papA

Producer and target organisms

Producer Organism: Pediococcus acidilactici [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaePediococcus

Target organismsPediococci - Lactobacilli - Leuconostoc - Brochothrix thermosphacta - propionibacteria - Bacilli - Enterococci - Staphylococci - Listeria clostridia - Listeria monocytogenes - Listeria innocua

Description

Mode of action:
Bactericidal activity (effective inhibitor of Listeria monocytogenes).

Uniprot and PDB links

UniProt Entry: P29430 P34912

PDB Entry5UKZ resolved by NMR

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=PAC-1.0; PLASMID=pSRQ11
MEDLINE=92384551;PubMed=1514784;
Marugg J.D., Gonzalez C.F., Kunka B.S., Ledeboer A.M., Pucci M.J., Toonen M.Y., Walker S.A., Zoetmulder L.C.M., Vandenbergh P.A.
"Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, and bacteriocin from Pediococcus acidilactici PAC1.0.", Appl. Environ. Microbiol. 58:2360-2367(1992).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=H; PLASMID=pSMB74
MEDLINE=94288967;PubMed=7764941;
Motlagh A.M., Bukhtiyarova M.B., Ray B.R.
"Complete nucleotide sequence of pSMB 74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici.", Lett. Appl. Microbiol. 18:305-312(1994).

[Ref. 3] | View abstract | Export citation

PROTEIN SEQUENCE OF 19-62, AND DISULFIDE BONDS, STRAIN=PAC-1.0; PLASMID=pSRQ11
MEDLINE=92246549;PubMed=1575516;
Henderson J.T., Chopko A.L., van Wassenaar P.D.
"Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0.", Arch. Biochem. Biophys. 295:5-12(1992).

[Ref. 4] | View abstract | Export citation

PROTEIN SEQUENCE OF 19-61.
MEDLINE=93019000;PubMed=1402795;
Lozano J.C.N., Meyer J.N., Sletten K., Pelaz C., Nes I.F.
"Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici.", J. Gen. Microbiol. 138:1985-1990(1992).

Gene structure

Gene id	Name	Description	Location
BACGene297	repB	RepB Orf319; involved in plasmid replication and maintenance; frequently found intheta-replicating plasmids from lactic acid bacteria	800..1759
BACGene298	repB	replication protein Orf192; involved in plasmid replication and maintenance; frequently found intheta-replicating plasmids from lactic acid bacteria; OrfX	1752..2330
BACGene299	papA	PapA	3271..3459
BACGene300	papB	PapB	3497..3835
BACGene301	papC	PapC	3859..4383
BACGene302	papD	PapD	4482..6656
BACGene303	papD	PemK-like protein Orf114b; plasmid maintenance system protein	complement(7247..7591)
BACGene304	papD	PemI-like protein Orf114a; plasmid maintenance system protein	complement(7585..7929)
BACGene305	papD	tyrosine recombinase Orf264; involved in plasmid replication and maintenance; frequently found intheta-replicating plasmids from lactic acid bacteria	7727..8521

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
KYYGNGVTCG KHSCSVDWGK ATTCIINNGA MAWATGGHQG NHKC

Wheel representation

3D Structure

Protein sequence annotations

Features of pediocin PA-1 (Pediocin ACH)

Feature	Position(s)	Length	Description
CHAIN	1↔44	44	Bacteriocin pediocin PA-1. Feature identifier = PRO_0000002746.
REGION	22↔34	13	Hydrophobic.
DISULFID	9↔14	6
DISULFID	24↔44	21

Composition

Hydrophobicity

Composition

Formula	C₁₉₆ H₂₉₇ N₆₁ O₆₀ S₅
Absent amino acids	EFLPR
Common amino acids	G
Mass (Da)	4646.95
Net charge	+6
Isoelectric point	8.67
Basic residues	7
Acidic residues	1
Hydrophobic residues	10
Polar residues	24
Aliphatic residues	4
Tiny residues	14
Boman Index	-49.55
Hydropathy Index	-0.49
Aliphatic Index	40
Instability Index	-0.48 (stable)
Half Life	Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	14230 M^-1 cm^-1
Absorbance 280nm	330.93

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