Bacteriocin: piscicolin 126
Accession: BAC081
Classification
Class: class IIA/YGNGV
Genetics
Gene: pisA
Producer and target organisms
Producer Organism: Carnobacterium piscicola [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium
Target organismsGram-positive bacteria: especially the food-borne pathogen Listeria monocytogenes - Carnobacterium - Enterococcus - Lactobacillus - Leuconostoc -
No effect on the growth of a number of yeasts & Gram-negative bacteria.
Description
Mode of action:
RANGE=19-62; NOTE=Ref.2.
RANGE=19-62; NOTE=Ref.2.
[Ref. 1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JG126
Gibbs G.M., Fitzgerald A., Selleck M.L., Harmark K., Hillier A.J., Davidson B.E.
"Characterization of the genes involved in the production of piscicolin 126, a bacteriocin from Carnobacterium piscicola JG126.", Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Gibbs G.M., Fitzgerald A., Selleck M.L., Harmark K., Hillier A.J., Davidson B.E.
"Characterization of the genes involved in the production of piscicolin 126, a bacteriocin from Carnobacterium piscicola JG126.", Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
PROTEIN SEQUENCE OF 19-62, AND MASS SPECTROMETRY, STRAIN=JG126
MEDLINE=96316406;PubMed=8702282;
Jack R.W., Wan J., Gordon J., Harmark K., Davidson B.E., Hillier A.J., Wettenhall R.E.H., Hickey M.W., Coventry J.M.
"Characterization of the chemical and antimicrobial properties of piscicolin 126, a bacteriocin produced by Carnobacterium piscicola JG126.", Appl. Environ. Microbiol. 62:2897-2903(1996).
MEDLINE=96316406;PubMed=8702282;
Jack R.W., Wan J., Gordon J., Harmark K., Davidson B.E., Hillier A.J., Wettenhall R.E.H., Hickey M.W., Coventry J.M.
"Characterization of the chemical and antimicrobial properties of piscicolin 126, a bacteriocin produced by Carnobacterium piscicola JG126.", Appl. Environ. Microbiol. 62:2897-2903(1996).
Loading...
Gene structure
| Gene id | Name | Description | Location |
| BACGene290 | pisI | putative piscicolin 126 immunity protein PisI | complement(1275..1571) |
| BACGene291 | pisA | piscicolin 126 protein PisA precursor cleaved after the double glycine upon translocation to yield the mature protein | complement(1813..2001) |
| BACGene292 | pisN | piscicolin 126 induction factor PisN precursor cleaved after the double glycine upon translocation to yield the mature protein | 2231..2374 |
| BACGene293 | pisK | putative histidine kinase PisK | 2420..3706 |
| BACGene294 | pisR | putative response regulator PisR | 3720..4466 |
| BACGene295 | pisT | putative ABC transporter PisT | 4496..6646 |
| BACGene296 | pisE | putative accessory protein PisE | 6664..7644 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVSCN KNGCTVDWSK AIGIIGNNAA ANLTTGGAAG WNKG |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔44 | 44 | Bacteriocin piscicolin-126. Feature identifier = PRO_0000002739. |
| DISULFID | 9↔14 | 6 | By similarity. |
Composition
Hydrophobicity
Composition
| Formula | C190
H295
N57
O61
S2 |
| Absent amino acids | EFHMPQR |
| Common amino acids | G |
| Mass (Da) | 4436.66 |
| Net charge | +3 |
| Isoelectric point | 9.33 |
| Basic residues | 4 |
| Acidic residues | 1 |
| Hydrophobic residues | 14 |
| Polar residues | 25 |
| Aliphatic residues | 6 |
| Tiny residues | 17 |
| Boman Index | -37.89 |
| Hydropathy Index | -0.31 |
| Aliphatic Index | 62.27 |
| Instability Index | 22.82 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 328.02 |
Users comments for piscicolin 126
No comments found