Bacteriocin: Enterocin 7A (Enterocin L50A)
Accession: BAC080
Classification
Genetics
Gene: mr10A; Synonyms=entNA
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Enterococcaceae
Enterococcus
Target organismsclostridia - propionibacteria - Listeria monocytogenes - Lactobacillus sakei - Enterococcus faecium - Pediococcus acidilactici - Pediococcus pentosaceus
Uniprot and PDB links
UniProt Entry: Q1A2D3
PDB Entry2M5Z resolved by NMR
NUCLEOTIDE SEQUENCE, STRAIN=6T1a
MEDLINE=99054929;PubMed=9835578;
Floriano B., Ruiz-Barba J.L., Jimenez-Diaz R.
"Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins.", Appl. Environ. Microbiol. 64:4883-4890(1998).
MEDLINE=99054929;PubMed=9835578;
Floriano B., Ruiz-Barba J.L., Jimenez-Diaz R.
"Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins.", Appl. Environ. Microbiol. 64:4883-4890(1998).
NUCLEOTIDE SEQUENCE, STRAIN=L50
MEDLINE=98215162;PubMed=9555877;
Cintas L.M., Casaus P., Holo H., Hernandez P.E., Nes I.F., Havarstein L.S.
"Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins.", J. Bacteriol. 180:1988-1994(1998).
MEDLINE=98215162;PubMed=9555877;
Cintas L.M., Casaus P., Holo H., Hernandez P.E., Nes I.F., Havarstein L.S.
"Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins.", J. Bacteriol. 180:1988-1994(1998).
NUCLEOTIDE SEQUENCE, STRAIN=MRR 10-3.
PubMed=16751538;DOI=10.1128/AEM.02940-05
Martin-Platero A.M., Valdivia E., Ruiz-Rodriguez M., Soler J.J., Martin-Vivaldi M., Maqueda M., Martinez-Bueno M.
"Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops).", Appl. Environ. Microbiol. 72:4245-4249(2006).
PubMed=16751538;DOI=10.1128/AEM.02940-05
Martin-Platero A.M., Valdivia E., Ruiz-Rodriguez M., Soler J.J., Martin-Vivaldi M., Maqueda M., Martinez-Bueno M.
"Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops).", Appl. Environ. Microbiol. 72:4245-4249(2006).
[Ref. 4]
NUCLEOTIDE SEQUENCE, STRAIN=N1-33.
Hata T., Alemu M., Kobayashi M., Tanaka R., Sato T., Suzuki C., Nitisinprasert S., Ohmomo S.
"Identification of enterocin N produced by Enterococcus faecalis N1-33 isolated from fermented vegetables in Thailand and its application to ready-to-eat foods as a biopreservative.", Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
Hata T., Alemu M., Kobayashi M., Tanaka R., Sato T., Suzuki C., Nitisinprasert S., Ohmomo S.
"Identification of enterocin N produced by Enterococcus faecalis N1-33 isolated from fermented vegetables in Thailand and its application to ready-to-eat foods as a biopreservative.", Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
[Ref. 5]
NUCLEOTIDE SEQUENCE, STRAIN=DBH18.
Jimenez J.J., Borrero J., Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB.", Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
Jimenez J.J., Borrero J., Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB.", Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
3D STRUCTURE
PubMed=23725536;DOI=10.1021/bi400359z
Lohans C.T., Towle K.M., Miskolzie M., McKay R.T., van Belkum M.J., McMullen L.M., Vederas J.C.
"Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide", Biochemistry 52: 3987-3994 (2013).
PubMed=23725536;DOI=10.1021/bi400359z
Lohans C.T., Towle K.M., Miskolzie M., McKay R.T., van Belkum M.J., McMullen L.M., Vederas J.C.
"Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide", Biochemistry 52: 3987-3994 (2013).
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Gene structure
Gene id | Name | Description | Location |
BACGene278 | hypothetical protein orf1 | 1..1830 | |
BACGene279 | enterocin JSA leader-less enterocin; entJSA | 2370..2504 | |
BACGene280 | enterocin JSB leader-less enterocin; entJSB | 2525..2656 | |
BACGene281 | hypothetical protein orf4 | 2877..3146 | |
BACGene282 | hypothetical protein orf5 | 3147..3416 | |
BACGene283 | hypothetical protein orf6 | 3420..3839 | |
BACGene284 | hypothetical protein orf7 | 3836..5212 | |
BACGene285 | hypothetical protein orf8 | 5222..5713 | |
BACGene286 | hypothetical protein orf9 | 5706..6926 | |
BACGene287 | hypothetical protein orf10 | 6923..7606 | |
BACGene288 | hypothetical protein orf11 | 7603..8802 | |
BACGene289 | hypothetical protein orf12 | 9758..11383 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | MGAIAKLVAK FGWPIVKKYY KQIMQFIGEG WAINKIIDWI KKHI |
Wheel representation
3D Structure
Protein sequence annotations
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔44 | 44 | Enterocin 7A.
Feature identifier = |
HELIX | 5↔8 | 4 | |
HELIX | 13↔16 | 4 | |
HELIX | 23↔27 | 5 | |
HELIX | 36↔43 | 8 |
Composition
Hydrophobicity
Composition
Formula | C252
H392
N60
O54
S2 |
Absent amino acids | CRST |
Common amino acids | I |
Mass (Da) | 5177.18 |
Net charge | +7 |
Isoelectric point | 10.68 |
Basic residues | 9 |
Acidic residues | 2 |
Hydrophobic residues | 21 |
Polar residues | 7 |
Aliphatic residues | 12 |
Tiny residues | 8 |
Boman Index | 3.34 |
Hydropathy Index | 0.202 |
Aliphatic Index | 110.91 |
Instability Index | 20.08 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 19480 M-1 cm-1 |
Absorbance 280nm | 453.02 |
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