Bacteriocin: Enterocin P
Accession: BAC079
Classification
Genetics
Gene: entP
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsLactobacillus sakei - Enterococcus faecium
Description
Enterococcus faecium L50 produces, in addition to EntL50, the sec-dependent pediocin-like enterocin P (EntP), and an unmodified non-pediocin-like bacteriocin, termed enterocin Q (EntQ), synthesized without an N-terminal leader sequence or signal peptide. Note=Ref2.
Classification note:
Enterocin P classification is problematic since it classified in class IIa, IIb and IIc according to literature. To review its classification, please see Zouhir et al., 2007.
Classification note:
Enterocin P classification is problematic since it classified in class IIa, IIb and IIc according to literature. To review its classification, please see Zouhir et al., 2007.
Uniprot and PDB links
UniProt Entry: O30434
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=L50
MEDLINE=20528333;PubMed=11073927;DOI=10.1128/JB.182.23.6806-6814.2000
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q.", J. Bacteriol. 182:6806-6814(2000).
MEDLINE=20528333;PubMed=11073927;DOI=10.1128/JB.182.23.6806-6814.2000
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q.", J. Bacteriol. 182:6806-6814(2000).
NUCLEOTIDE SEQUENCE, STRAIN=P13
MEDLINE=98027370;PubMed=9361419;
Cintas L.M., Casaus P., Havarstein L.S., Hernandez P.E., Nes I.F.
"Biochemical and genetic characterization of enterocin P, a novel sec- dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum.", Appl. Environ. Microbiol. 63:4321-4330(1997).
MEDLINE=98027370;PubMed=9361419;
Cintas L.M., Casaus P., Havarstein L.S., Hernandez P.E., Nes I.F.
"Biochemical and genetic characterization of enterocin P, a novel sec- dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum.", Appl. Environ. Microbiol. 63:4321-4330(1997).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene276 | entP | enterocin P precursor broad antimicrobial spectrum pediocin-like bacteriocin secreted by the generalsecretory pathway; sec-dependent bacteriocin | 173..388 |
| BACGene277 | entP | putative bacteriocin immunity protein orf2 | 393..659 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ATRSYGNGVY CNNSKCWVNW GEAKENIAGI VISGWASGLA GMGH |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔44 | 44 | Bacteriocin enterocin-P. Feature identifier = PRO_5000053228. |
| DISULFID | 11↔16 | 6 | By similarity. |
Composition
Hydrophobicity
Composition
| Formula | C202
H303
N59
O61
S3 |
| Absent amino acids | DFPQ |
| Common amino acids | G |
| Mass (Da) | 4648.91 |
| Net charge | +2 |
| Isoelectric point | 8.22 |
| Basic residues | 4 |
| Acidic residues | 2 |
| Hydrophobic residues | 15 |
| Polar residues | 22 |
| Aliphatic residues | 7 |
| Tiny residues | 17 |
| Boman Index | -33.68 |
| Hydropathy Index | -0.15 |
| Aliphatic Index | 66.59 |
| Instability Index | 13.31 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 19605 M-1 cm-1 |
| Absorbance 280nm | 455.93 |
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