Bacteriocin: sakacin-P (Sakacin 674)

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Bacteriocin: sakacin-P (Sakacin 674)

Accession: BAC078

Classification
Genetics
Gene: sakP; Synonyms=sakR, sppA
Uniprot and PDB links
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LTH673
MEDLINE=94236240;PubMed=8180701;
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673.", Microbiology 140:361-367(1994).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-61, AND CHARACTERIZATION, STRAIN=Lb674
MEDLINE=94186010;PubMed=8138128;DOI=10.1016/0378-1097(94)90005-1
Holck A.L., Axelsson L., Huehne K., Kroeckel L.
"Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake Lb674.", FEMS Microbiol. Lett. 115:143-150(1994).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb674
MEDLINE=96262715;PubMed=8704983;
Huehne K., Axelsson L., Holck A., Kroeckel L.
"Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strains.", Microbiology 142:1437-1448(1996).
PROTEIN SEQUENCE OF 19-59, AND CHARACTERIZATION, STRAIN=LTH673
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).
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Gene structure
genome of sakacin-P (Sakacin 674)

Gene idNameDescriptionLocation
BACGene270sppKSppK putative histidine protein kinase572..1918
BACGene271sppRSppR putative response regulator protein ttg start codon1920..2666
BACGene272sppASppA bacteriocin preprotein2813..2998
BACGene273spiASpiA putative protein conferring immunity to sakacin P3092..3388
BACGene274sppTSppT putative ATP-dependent translocator protein3739..5895
BACGene275sppESppE putative accessory protein for sakacin P export5910..7286
sppKsppRsppAspiAsppTsppE
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 KYYGNGVHCG KHSCTVDWGT AIGNIGNNAA ANWATGGNAG WNK

Wheel representation
wheel representation of sakacin-P (Sakacin 674)
3D Structure
Protein sequence annotations
Features of sakacin-P (Sakacin 674)


FeaturePosition(s)LengthDescription
CHAIN1↔4343
Bacteriocin sakacin-P.
Feature identifier = PRO_0000002752.
DISULFID9↔146
STRAND2↔43
STRAND10↔123
HELIX19↔3214
TURN35↔417
Composition
Hydrophobicity

Composition
Formula C192 H280 N60 O59 S2
Absent amino acids EFLMPQR
Common amino acids G
Mass (Da) 4455.57
Net charge +4
Isoelectric point 8.77
Basic residues 5
Acidic residues 1
Hydrophobic residues 13
Polar residues 24
Aliphatic residues 4
Tiny residues 16
Boman Index -45.77
Hydropathy Index -0.57
Aliphatic Index 45.58
Instability Index 21.03 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 19605 M-1 cm-1
Absorbance 280nm 466.79

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