Bacteriocin: sakacin-P (Sakacin 674)
Accession: BAC078
Classification
Class: class IIA/YGNGV
Genetics
Gene: sakP; Synonyms=sakR, sppA
Producer and target organisms
Producer Organism: Lactobacillus sakei [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsLactobacilli - Carnobacteria - Enterococcus faecalis - Brochothrix thermosphacta - Listeria monocytogenes - Listeria innocua - Listeria ivanovii
Uniprot and PDB links
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LTH673
MEDLINE=94236240;PubMed=8180701;
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673.", Microbiology 140:361-367(1994).
MEDLINE=94236240;PubMed=8180701;
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673.", Microbiology 140:361-367(1994).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-61, AND CHARACTERIZATION, STRAIN=Lb674
MEDLINE=94186010;PubMed=8138128;DOI=10.1016/0378-1097(94)90005-1
Holck A.L., Axelsson L., Huehne K., Kroeckel L.
"Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake Lb674.", FEMS Microbiol. Lett. 115:143-150(1994).
MEDLINE=94186010;PubMed=8138128;DOI=10.1016/0378-1097(94)90005-1
Holck A.L., Axelsson L., Huehne K., Kroeckel L.
"Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake Lb674.", FEMS Microbiol. Lett. 115:143-150(1994).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb674
MEDLINE=96262715;PubMed=8704983;
Huehne K., Axelsson L., Holck A., Kroeckel L.
"Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strains.", Microbiology 142:1437-1448(1996).
MEDLINE=96262715;PubMed=8704983;
Huehne K., Axelsson L., Holck A., Kroeckel L.
"Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strains.", Microbiology 142:1437-1448(1996).
[Ref. 4]
PROTEIN SEQUENCE OF 19-59, AND CHARACTERIZATION, STRAIN=LTH673
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).
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Gene structure
Gene id | Name | Description | Location |
BACGene270 | sppK | SppK putative histidine protein kinase | 572..1918 |
BACGene271 | sppR | SppR putative response regulator protein ttg start codon | 1920..2666 |
BACGene272 | sppA | SppA bacteriocin preprotein | 2813..2998 |
BACGene273 | spiA | SpiA putative protein conferring immunity to sakacin P | 3092..3388 |
BACGene274 | sppT | SppT putative ATP-dependent translocator protein | 3739..5895 |
BACGene275 | sppE | SppE putative accessory protein for sakacin P export | 5910..7286 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVHCG KHSCTVDWGT AIGNIGNNAA ANWATGGNAG WNK |
Wheel representation
3D Structure
Protein sequence annotations
Feature | Position(s) | Length | Description |
CHAIN | 1↔43 | 43 | Bacteriocin sakacin-P. Feature identifier = PRO_0000002752. |
DISULFID | 9↔14 | 6 | |
STRAND | 2↔4 | 3 | |
STRAND | 10↔12 | 3 | |
HELIX | 19↔32 | 14 | |
TURN | 35↔41 | 7 |
Composition
Hydrophobicity
Composition
Formula | C192
H280
N60
O59
S2 |
Absent amino acids | EFLMPQR |
Common amino acids | G |
Mass (Da) | 4455.57 |
Net charge | +4 |
Isoelectric point | 8.77 |
Basic residues | 5 |
Acidic residues | 1 |
Hydrophobic residues | 13 |
Polar residues | 24 |
Aliphatic residues | 4 |
Tiny residues | 16 |
Boman Index | -45.77 |
Hydropathy Index | -0.57 |
Aliphatic Index | 45.58 |
Instability Index | 21.03 (stable) |
Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
Extinction Coefficient | 19605 M-1 cm-1 |
Absorbance 280nm | 466.79 |
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