Bacteriocin: thermophilin 13

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Bacteriocin: thermophilin 13

Accession: BAC074

Classification
Class: Unclassified
Genetics
Gene: thermophilin 13 thmB
Uniprot and PDB links

UniProt Entry: O54455

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=SFi13
MEDLINE=97306337;PubMed=9162062;DOI=10.1074/jbc.272.22.14277
Marciset O., Jeronimus-Stratingh M.C., Mollet B., Poolman B.
"Thermophilin 13, a nontypical antilisterial poration complex bacteriocin, that functions without a receptor.", J. Biol. Chem. 272:14277-14284(1997).
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Gene structure
genome of thermophilin 13

Gene idNameDescriptionLocation
BACGene249thmAamphipathic pore-forming peptide precursor ThmA79..336
BACGene250thmBenhancer peptide for thermophilin 13 precursor requires ThmB for full activity353..547
BACGene251thermophilin 13 operonunknown hydrophobic peptide537..695
thmAthmBthermophilin 13 operon
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 QINWGSVVGH CIGGAIIGGA FSGGAAAGVG CLVGSGKAII NGL

Wheel representation
wheel representation of thermophilin 13
Protein sequence annotations
Features of thermophilin 13


FeaturePosition(s)LengthDescription
CHAIN1↔4343
enhancer peptide for thermophilin 13.
Feature identifier = PRO_5000145346.
Composition
Hydrophobicity

Composition
Formula C172 H278 N50 O50 S2
Absent amino acids DEMPRTY
Common amino acids G
Mass (Da) 3929.3
Net charge +2
Isoelectric point 8.24
Basic residues 2
Acidic residues 0
Hydrophobic residues 20
Polar residues 20
Aliphatic residues 12
Tiny residues 22
Boman Index 47.24
Hydropathy Index 1.02
Aliphatic Index 113.49
Instability Index 0.72 (stable)
Half Life Mammalian : 0.8 hour
Yeast : 10 min
E. coli : >10 hour
Extinction Coefficient 5625 M-1 cm-1
Absorbance 280nm 133.93

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