Bacteriocin: microcin B17 (MccB17)
Accession: BAC072
Classification
Class: Unclassified
Genetics
Gene: mcbA
Producer and target organisms
Producer Organism: Escherichia coli [Gram-negative]
Taxonomy: Bacteria
ProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Target organismsUnavailable data
Description
Mode of action:
This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.
post-translational modification:
The processed N-terminus does not resemble a typical secretion signal sequence.
The Cys residues as well as some Gly and Ser are post- translationally modified. Modifications include the formation of four thiazole and four oxazole rings that result, respectively, from the condensation of four cysteine and four serine side chains with the carbonyl group of the preceding amino acid.
This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.
post-translational modification:
The processed N-terminus does not resemble a typical secretion signal sequence.
The Cys residues as well as some Gly and Ser are post- translationally modified. Modifications include the formation of four thiazole and four oxazole rings that result, respectively, from the condensation of four cysteine and four serine side chains with the carbonyl group of the preceding amino acid.
Uniprot and PDB links
PDB Entry2MLP resolved by NMR
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=88217867;PubMed=3329729;DOI=10.1002/prot.340010305
Davagnino J., Herrero M., Furlong D., Moreno F., Kolter R.
"The DNA replication inhibitor microcin B17 is a forty-three-amino- acid protein containing sixty percent glycine.", Proteins 1:230-238(1986).
MEDLINE=88217867;PubMed=3329729;DOI=10.1002/prot.340010305
Davagnino J., Herrero M., Furlong D., Moreno F., Kolter R.
"The DNA replication inhibitor microcin B17 is a forty-three-amino- acid protein containing sixty percent glycine.", Proteins 1:230-238(1986).
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=89123111;PubMed=2644225;
Genilloud O., Moreno F., Kolter R.
"DNA sequence, products, and transcriptional pattern of the genes involved in production of the DNA replication inhibitor microcin B17.", J. Bacteriol. 171:1126-1135(1989).
MEDLINE=89123111;PubMed=2644225;
Genilloud O., Moreno F., Kolter R.
"DNA sequence, products, and transcriptional pattern of the genes involved in production of the DNA replication inhibitor microcin B17.", J. Bacteriol. 171:1126-1135(1989).
[Ref. 4]
PARTIAL PROTEIN SEQUENCE OF 27-69.
Bayer A., Stevanovic S., Freund S., Metzger J.W., Jung G.
"Isolation and structure elucidation of the 43-peptide antibiotic microcin B17.",
Bayer A., Stevanovic S., Freund S., Metzger J.W., Jung G.
"Isolation and structure elucidation of the 43-peptide antibiotic microcin B17.",
PROTEIN SEQUENCE OF 27-38, AND POST-TRANSLATONAL MODIFICATIONS.
MEDLINE=94240167;PubMed=8183941;
Yorgey P., Lee J., Koerdel J., Vivas E., Warner P., Jebaratnam D., Kolter R.
"Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor.", Proc. Natl. Acad. Sci. U.S.A. 91:4519-4523(1994).
MEDLINE=94240167;PubMed=8183941;
Yorgey P., Lee J., Koerdel J., Vivas E., Warner P., Jebaratnam D., Kolter R.
"Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor.", Proc. Natl. Acad. Sci. U.S.A. 91:4519-4523(1994).
FUNCTION.
MEDLINE=91122055;PubMed=1846808;
Vizan J.L., Hernandez-Chico C., del Castillo I., Moreno F.
"The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.", EMBO J. 10:467-476(1991).
MEDLINE=91122055;PubMed=1846808;
Vizan J.L., Hernandez-Chico C., del Castillo I., Moreno F.
"The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.", EMBO J. 10:467-476(1991).
STRUCTURE BY NMR OF 1-26.
MEDLINE=98213789;PubMed=9545435;DOI=10.1016/S1074-5521(98)90635-4
Roy R.S., Kim S., Baleja J.D., Walsh C.T.
"Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.", Chem. Biol. 5:217-228(1998).
MEDLINE=98213789;PubMed=9545435;DOI=10.1016/S1074-5521(98)90635-4
Roy R.S., Kim S., Baleja J.D., Walsh C.T.
"Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.", Chem. Biol. 5:217-228(1998).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene244 | mcbA | microcin subunit | 408..617 |
| BACGene245 | mcbB | microcin subunit | 643..1530 |
| BACGene246 | mcbC | microcin subunit | 1532..2350 |
| BACGene247 | mcbD | microcin subunit | 2331..3521 |
| BACGene248 | imm | 3531..3810 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | VGIGGGGGGG GGGSCGGQGG GCGGCSNGCS GGNGGSGGSG SHI |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | Bacteriocin microcin B17. Feature identifier = PRO_0000002773. |
| Cross-link | 13↔14 | 2 | Oxazole-4-carboxylic acid (Gly-Ser). |
| Cross-link | 14↔15 | 2 | Thiazole-4-carboxylic acid (Ser-Cys). |
| Cross-link | 21↔22 | 2 | Thiazole-4-carboxylic acid (Gly-Cys). |
| Cross-link | 24↔25 | 2 | Thiazole-4-carboxylic acid (Gly-Cys). |
| Cross-link | 28↔29 | 2 | Thiazole-4-carboxylic acid (Gly-Cys). |
| Cross-link | 29↔30 | 2 | Oxazole-4-carboxylic acid (Cys-Ser). |
| Cross-link | 35↔36 | 2 | Oxazole-4-carboxylic acid (Gly-Ser). |
| Cross-link | 38↔39 | 2 | Oxazole-4-carboxylic acid (Gly-Ser). |
Composition
Hydrophobicity
Composition
| Formula | C118
H188
N48
O53
S4 |
| Absent amino acids | ADEFKLMPRTWY |
| Common amino acids | G |
| Mass (Da) | 3274.06 |
| Net charge | +1 |
| Isoelectric point | 7.12 |
| Basic residues | 1 |
| Acidic residues | 0 |
| Hydrophobic residues | 3 |
| Polar residues | 38 |
| Aliphatic residues | 3 |
| Tiny residues | 32 |
| Boman Index | -0.44 |
| Hydropathy Index | -0.13 |
| Aliphatic Index | 24.88 |
| Instability Index | 60.57 (unstable) |
| Half Life |
Mammalian : 100 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 250 M-1 cm-1 |
| Absorbance 280nm | 5.95 |
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