Bacteriocin: microcin B17 (MccB17)

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Bacteriocin: microcin B17 (MccB17)

Accession: BAC072

Classification
Class: Unclassified
Genetics
Gene: mcbA
Producer and target organisms
Description
Mode of action:
This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.

post-translational modification:
The processed N-terminus does not resemble a typical secretion signal sequence.

The Cys residues as well as some Gly and Ser are post- translationally modified. Modifications include the formation of four thiazole and four oxazole rings that result, respectively, from the condensation of four cysteine and four serine side chains with the carbonyl group of the preceding amino acid.
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=88217867;PubMed=3329729;DOI=10.1002/prot.340010305
Davagnino J., Herrero M., Furlong D., Moreno F., Kolter R.
"The DNA replication inhibitor microcin B17 is a forty-three-amino- acid protein containing sixty percent glycine.", Proteins 1:230-238(1986).
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=89123111;PubMed=2644225;
Genilloud O., Moreno F., Kolter R.
"DNA sequence, products, and transcriptional pattern of the genes involved in production of the DNA replication inhibitor microcin B17.", J. Bacteriol. 171:1126-1135(1989).
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
MEDLINE=88216163;PubMed=2835580;
Conell N., Han Z., Moreno F., Kolter R.
"An E. coli promoter induced by the cessation of growth.", Mol. Microbiol. 1:195-201(1987).
PARTIAL PROTEIN SEQUENCE OF 27-69.
Bayer A., Stevanovic S., Freund S., Metzger J.W., Jung G.
"Isolation and structure elucidation of the 43-peptide antibiotic microcin B17.",
PROTEIN SEQUENCE OF 27-38, AND POST-TRANSLATONAL MODIFICATIONS.
MEDLINE=94240167;PubMed=8183941;
Yorgey P., Lee J., Koerdel J., Vivas E., Warner P., Jebaratnam D., Kolter R.
"Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor.", Proc. Natl. Acad. Sci. U.S.A. 91:4519-4523(1994).
FUNCTION.
MEDLINE=91122055;PubMed=1846808;
Vizan J.L., Hernandez-Chico C., del Castillo I., Moreno F.
"The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.", EMBO J. 10:467-476(1991).
STRUCTURE BY NMR OF 1-26.
MEDLINE=98213789;PubMed=9545435;DOI=10.1016/S1074-5521(98)90635-4
Roy R.S., Kim S., Baleja J.D., Walsh C.T.
"Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.", Chem. Biol. 5:217-228(1998).
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End Note
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Gene structure
genome of microcin B17  (MccB17)

Gene idNameDescriptionLocation
BACGene244mcbAmicrocin subunit408..617
BACGene245mcbBmicrocin subunit643..1530
BACGene246mcbCmicrocin subunit1532..2350
BACGene247mcbDmicrocin subunit2331..3521
BACGene248imm3531..3810
mcbAmcbBmcbCmcbDimm
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 VGIGGGGGGG GGGSCGGQGG GCGGCSNGCS GGNGGSGGSG SHI

Wheel representation
wheel representation of microcin B17  (MccB17)
3D Structure
Protein sequence annotations
Features of microcin B17  (MccB17)


FeaturePosition(s)LengthDescription
CHAIN1↔4343
Bacteriocin microcin B17.
Feature identifier = PRO_0000002773.
Cross-link13↔142Oxazole-4-carboxylic acid (Gly-Ser).
Cross-link14↔152Thiazole-4-carboxylic acid (Ser-Cys).
Cross-link21↔222Thiazole-4-carboxylic acid (Gly-Cys).
Cross-link24↔252Thiazole-4-carboxylic acid (Gly-Cys).
Cross-link28↔292Thiazole-4-carboxylic acid (Gly-Cys).
Cross-link29↔302Oxazole-4-carboxylic acid (Cys-Ser).
Cross-link35↔362Oxazole-4-carboxylic acid (Gly-Ser).
Cross-link38↔392Oxazole-4-carboxylic acid (Gly-Ser).
Composition
Hydrophobicity

Composition
Formula C118 H188 N48 O53 S4
Absent amino acids ADEFKLMPRTWY
Common amino acids G
Mass (Da) 3274.06
Net charge +1
Isoelectric point 7.12
Basic residues 1
Acidic residues 0
Hydrophobic residues 3
Polar residues 38
Aliphatic residues 3
Tiny residues 32
Boman Index -0.44
Hydropathy Index -0.13
Aliphatic Index 24.88
Instability Index 60.57 (unstable)
Half Life Mammalian : 100 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 5.95

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