Bacteriocin: sakacin-A
Accession: BAC066
Classification
Class: class IIA/YGNGV
Genetics
Gene: sapA; Synonyms=sakA
Producer and target organisms
Producer Organism: Lactobacillus sakei [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsinhibits closely related Lactobacilli - Enterococci - Listeria monocytogenes - Listeria ivanovii - Enterococcus faecalis - Carnobacterium sp - Brochothrix thermosphacta
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE, STRAIN=Lb706
MEDLINE=93139766;PubMed=1487735;
Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.
"Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Gen. Microbiol. 138:2715-2720(1992).
MEDLINE=93139766;PubMed=1487735;
Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.
"Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Gen. Microbiol. 138:2715-2720(1992).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb706
MEDLINE=95238285;PubMed=7721704;
Axelsson L., Holck A.
"The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Bacteriol. 177:2125-2137(1995).
MEDLINE=95238285;PubMed=7721704;
Axelsson L., Holck A.
"The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Bacteriol. 177:2125-2137(1995).
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Gene structure
Gene id | Name | Description | Location |
BACGene231 | orf1 | complement(5..268) | |
BACGene232 | saiA | sakacin A immunity factor start codon ttg | complement(653..925) |
BACGene233 | sapA | sakacin A precursor previously designated sakA | complement(940..1119) |
BACGene234 | orf4 | 2526..2663 | |
BACGene235 | sapK | histidine kinase putative; previously designated sakB | 2724..4010 |
BACGene236 | sapR | response regulator putative | 4026..4769 |
BACGene237 | sapT | ABC exporter putative | 4842..7001 |
BACGene238 | sapE | accessory factor for ABC exporter putative | 7020..8405 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | ARSYGNGVYC NNKKCWVNRG EATQSIIGGM ISGWASGLAG M |
Wheel representation
Protein sequence annotations
Feature | Position(s) | Length | Description |
CHAIN | 1↔41 | 41 | Bacteriocin sakacin-A. Feature identifier = PRO_0000002750. |
DISULFID | 10↔15 | 6 | By similarity. |
Composition
Hydrophobicity
Composition
Formula | C184
H288
N56
O56
S4 |
Absent amino acids | DFHP |
Common amino acids | G |
Mass (Da) | 4327.58 |
Net charge | +3 |
Isoelectric point | 9.37 |
Basic residues | 4 |
Acidic residues | 1 |
Hydrophobic residues | 12 |
Polar residues | 21 |
Aliphatic residues | 6 |
Tiny residues | 16 |
Boman Index | -41.86 |
Hydropathy Index | -0.19 |
Aliphatic Index | 61.95 |
Instability Index | 27.63 (stable) |
Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 14105 M-1 cm-1 |
Absorbance 280nm | 352.63 |
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