Bacteriocin: sakacin-A

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Bacteriocin: sakacin-A

Accession: BAC066

Classification

Class: class IIA/YGNGV

Genetics

Gene: sapA; Synonyms=sakA

Producer and target organisms

Producer Organism: Lactobacillus sakei [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsinhibits closely related Lactobacilli - Enterococci - Listeria monocytogenes - Listeria ivanovii - Enterococcus faecalis - Carnobacterium sp - Brochothrix thermosphacta

Related Entries

sakacin-P (Sakacin 674)Sakacin G

Uniprot and PDB links

UniProt Entry: P0A310 P35619

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE, STRAIN=Lb706
MEDLINE=93139766;PubMed=1487735;
Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.
"Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Gen. Microbiol. 138:2715-2720(1992).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=Lb706
MEDLINE=95238285;PubMed=7721704;
Axelsson L., Holck A.
"The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.", J. Bacteriol. 177:2125-2137(1995).

Gene structure

Gene id	Name	Description	Location
BACGene231	orf1		complement(5..268)
BACGene232	saiA	sakacin A immunity factor start codon ttg	complement(653..925)
BACGene233	sapA	sakacin A precursor previously designated sakA	complement(940..1119)
BACGene234	orf4		2526..2663
BACGene235	sapK	histidine kinase putative; previously designated sakB	2724..4010
BACGene236	sapR	response regulator putative	4026..4769
BACGene237	sapT	ABC exporter putative	4842..7001
BACGene238	sapE	accessory factor for ABC exporter putative	7020..8405

Protein sequence

........10 ........20 ........30 ........40 ........50
| | | | |
ARSYGNGVYC NNKKCWVNRG EATQSIIGGM ISGWASGLAG M

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔41	41	Bacteriocin sakacin-A. Feature identifier = PRO_0000002750.
DISULFID	10↔15	6	By similarity.

Composition

Hydrophobicity

Composition

Formula	C₁₈₄ H₂₈₈ N₅₆ O₅₆ S₄
Absent amino acids	DFHP
Common amino acids	G
Mass (Da)	4327.58
Net charge	+3
Isoelectric point	9.37
Basic residues	4
Acidic residues	1
Hydrophobic residues	12
Polar residues	21
Aliphatic residues	6
Tiny residues	16
Boman Index	-41.86
Hydropathy Index	-0.19
Aliphatic Index	61.95
Instability Index	27.63 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	14105 M^-1 cm^-1
Absorbance 280nm	352.63

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