Bacteriocin: sublancin 168

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Bacteriocin: sublancin 168

Accession: BAC062

Classification
Class: Lantibiotic
Genetics
Gene: sunA; OrderedLocusNames=BSU21480
Producer and target organisms

Producer Organism: Bacillus subtilis [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesBacillaceaeBacillus

Target organismsactive on some Gram-positive bacteria

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. Inhibits Bacillus cereus spore outgrowth, after the germination stage, approximately 1000-fold better than it inhibits exponential growth of the same cells.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor, both of which are probably performed by sunT. Production of active sublancin 168 requires at least one thiol-disulfide oxidoreductase (bdbB or in its absence bdbC). NOTE=Ref.1. greater than that expected from the amino acid composition, making it likely that there is an unidentified posttranslational modification of the peptide.
Uniprot and PDB links

UniProt Entry: P68577 O34781 O64033

PDB Entry2MIJ resolved by NMR

NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-37, MASS SPECTROMETRY, AND CHARACTERIZATION OF ANTIBIOTIC ACTIVITY, STRAIN=168 / BR151
MEDLINE=98389743;PubMed=9722542;DOI=10.1074/jbc.273.36.23134
Paik S.H., Chakicherla A., Hansen J.N.
"Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168.", J. Biol. Chem. 273:23134-23142(1998).
REQUIREMENT FOR SUNT, AND BDBB OR BDBC FOR ANTIBIOTIC PRODUCTION, STRAIN=168
MEDLINE=21988147;PubMed=11872755;DOI=10.1074/jbc.M201158200
Dorenbos R., Stein T., Kabel J., Bruand C., Bolhuis A., Bron S., Quax W.J., Van Dijl J.M.
"Thiol-disulfide oxidoreductases are essential for the production of the lantibiotic sublancin 168.", J. Biol. Chem. 277:16682-16688(2002).
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End Note
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BibTeX
Gene structure
genome of sublancin 168

Gene idNameDescriptionLocation
BACGene225sunSsublancin glycosyltransferase 16.6: Maintain 16.4: Excrete Evidence 1a: Function experimentally demonstrated in the studied strain;PubMedId: 15743949, 21196935, 21910430; Product type e: enzymecomplement(2265668..2266936)
BACGene226sunTsublancin 168 lantibiotic transporter 16.1: Circulate Evidence 1a: Function experimentally demonstrated in the studied strain;PubMedId: 15743949, 9722542; Product type t: transportercomplement(2267346..2269463)
BACGene227sunAsublancin 168 lantibiotic antimicrobial precursor peptide in SPBeta prophage 16.5: Explore 16.8: Protect Evidence 1a: Function experimentally demonstrated in the studied strain;PubMedId: 15743949, 9722542; Product type h: extrachromosomalcomplement(2269521..2269691)
BACGene228sunIprotein of immunity to sublancin 16.5: Explore 16.8: Protect Evidence 1a: Function experimentally demonstrated in the studied strain;PubMedId: 10376821, 19047653; Product type cp: cell processcomplement(2269988..2270305)
sunSsunTsunAsunI
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 GLGKAQCAAL WLQCASGGTI GCGGGAVACQ NYRQFCR

Wheel representation
wheel representation of sublancin 168
3D Structure
Protein sequence annotations
Features of sublancin 168


FeaturePosition(s)LengthDescription
PEPTIDE1↔3737
SPBc2 prophage-derived bacteriocinsublancin-168.
Feature identifier = PRO_0000017145.
CARBOHYD22S-linked (Glc).
DISULFID7↔3630
DISULFID14↔2916
MUTAGEN21G->E: No effect on glycosylation.
MUTAGEN21G->Q: No effect on glycosylation; whenassociated with A-42.
MUTAGEN22C->S: Abolishes glycosylation.
MUTAGEN23G->A: No effect on glycosylation; whenassociated with Q-40.
MUTAGEN23G->E,K: No effect on glycosylation.
Composition
Hydrophobicity

Composition
Formula C156 H248 N50 O46 S5
Absent amino acids DEHMP
Common amino acids G
Mass (Da) 3738.96
Net charge +3
Isoelectric point 8.51
Basic residues 3
Acidic residues 0
Hydrophobic residues 13
Polar residues 17
Aliphatic residues 5
Tiny residues 15
Boman Index -16.49
Hydropathy Index 0.24
Aliphatic Index 66.22
Instability Index 35.76 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 7240 M-1 cm-1
Absorbance 280nm 201.11

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