Bacteriocin: leucocin-A (Leucocin A-UAL 187)
Accession: BAC058
Classification
Class: class IIA/YGNGV
Genetics
Gene: lcnA
Producer and target organisms
Producer Organism: Leuconostoc gelidum [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLeuconostoc
Target organismsLactic bacteria - Listeria monocytogenes
Description
Leuconostoc mesenteroides TA33a produces three bacteriocins leucocins A-, B- and C. Leucocin A-TA33a, is identical to leucocin A-UAL 187. Circular dichroism spectra of the leucocins in aqueous solution and micellar SDS indicated that they undergo a structural transition when in a membrane-mimicking environment. Theoretical predictions from circular dichroism data suggest that leucocin A adopt a beta-structure (48%) in membrane-mimicking environments.
Uniprot and PDB links
UniProt Entry: P34034
PDB Entry1CW6 resolved by NMR 2LEU resolved by NMR 2LJQ resolved by NMR 2LJT resolved by NMR 3LEU resolved by NMR
STRUCTURE BY NMR.
MEDLINE=93120109;PubMed=8418850;
Sailer M., Helms G.L., Henkel T., Niemczura W.P., Stiles M.E., Vederas J.C.
"15N- and 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.", Biochemistry 32:310-318(1993).
MEDLINE=93120109;PubMed=8418850;
Sailer M., Helms G.L., Henkel T., Niemczura W.P., Stiles M.E., Vederas J.C.
"15N- and 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.", Biochemistry 32:310-318(1993).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 25-37, STRAIN=UAL 187
MEDLINE=92041660;PubMed=1840587;
Hastings J.W., Sailer M., Johnson K., Roy K.L., Vederas J.C., Stiles M.E.
"Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum.", J. Bacteriol. 173:7491-7500(1991).
MEDLINE=92041660;PubMed=1840587;
Hastings J.W., Sailer M., Johnson K., Roy K.L., Vederas J.C., Stiles M.E.
"Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum.", J. Bacteriol. 173:7491-7500(1991).
STRUCTURE BY NMR.
MEDLINE=98060758;PubMed=9398233;DOI=10.1021/bi971263h
Gallagher N.L.F., Sailer M., Niemczura W.P., Nakashima T.T., Stiles M.E., Vederas J.C.
"Three-dimensional structure of leucocin A in trifluoroethanol and dodecylphosphocholine micelles: spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria.", Biochemistry 36:15062-15072(1997).
MEDLINE=98060758;PubMed=9398233;DOI=10.1021/bi971263h
Gallagher N.L.F., Sailer M., Niemczura W.P., Nakashima T.T., Stiles M.E., Vederas J.C.
"Three-dimensional structure of leucocin A in trifluoroethanol and dodecylphosphocholine micelles: spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria.", Biochemistry 36:15062-15072(1997).
PROTEIN SEQUENCE, STRAIN=QU 15
PubMed=20070442;DOI=10.1111/j.1365-2672.2009.04653.x
Sawa N, Okamura K, Zendo T, Himeno K, Nakayama J, Sonomoto K.
"Identification and characterization of novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15", Journal of Applied Microbiology (2010).
PubMed=20070442;DOI=10.1111/j.1365-2672.2009.04653.x
Sawa N, Okamura K, Zendo T, Himeno K, Nakayama J, Sonomoto K.
"Identification and characterization of novel multiple bacteriocins produced by Leuconostoc pseudomesenteroides QU 15", Journal of Applied Microbiology (2010).
STRUCTURE BY NMR, STRAIN=UAL 187.
PubMed=22121114;DOI=10.1002/cbic.201100634
Sit C.S., Lohans C.T., van Belkum M.J., Campbell C.D., Miskolzie M., Vederas J.C.
"Substitution of a conserved disulfide in the type IIa bacteriocin, leucocin A, with L-leucine and L-serine residues: effects on activity and three-dimensional structure.", Chembiochem 13: 35-38 (2012).
PubMed=22121114;DOI=10.1002/cbic.201100634
Sit C.S., Lohans C.T., van Belkum M.J., Campbell C.D., Miskolzie M., Vederas J.C.
"Substitution of a conserved disulfide in the type IIa bacteriocin, leucocin A, with L-leucine and L-serine residues: effects on activity and three-dimensional structure.", Chembiochem 13: 35-38 (2012).
Loading...
Gene structure
| Gene id | Name | Description | Location |
| BACGene216 | lcnA | leucocin A | 306..491 |
| BACGene217 | lcnA | leucocin A immunity protein | 512..853 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | KYYGNGVHCT KSGCSVNWGE AFSAGVHRLA NGGNGFW |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔37 | 37 | Bacteriocin leucocin-A. Feature identifier = PRO_0000002743. |
| DISULFID | 9↔14 | 6 | |
| STRAND | 4↔6 | 3 | |
| STRAND | 8↔10 | 3 | |
| STRAND | 13↔15 | 3 | |
| HELIX | 19↔29 | 11 | |
| STRAND | 33↔35 | 3 |
Composition
Hydrophobicity
Composition
| Formula | C174
H248
N52
O50
S2 |
| Absent amino acids | DIMPQ |
| Common amino acids | G |
| Mass (Da) | 3950.93 |
| Net charge | +4 |
| Isoelectric point | 8.77 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 11 |
| Polar residues | 20 |
| Aliphatic residues | 4 |
| Tiny residues | 14 |
| Boman Index | -38.59 |
| Hydropathy Index | -0.39 |
| Aliphatic Index | 42.16 |
| Instability Index | -1.5 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 391.81 |
Users comments for leucocin-A (Leucocin A-UAL 187)
No comments found