Bacteriocin: lactocin-S

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Bacteriocin: lactocin-S

Accession: BAC057

Classification

Class: Lantibiotic

Genetics

Gene: lasA

Producer and target organisms

Producer Organism: Lactobacillus sakei L45 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsLeuconostoc - Carnobacteria - Lactobacilli - Pediococci - Lactococci - Listeria monocytogenes - Listeria innocua - Staphylococcus - Bacillus cereus - Clostridium spp

Description

Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.

Related Entries

lactocin-705

Uniprot and PDB links

UniProt Entry: P23826 Q48848 Q48858

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=95050447;PubMed=7961627;
Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F.
"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide.", J. Biol. Chem. 269:27183-27185(1994).

[Ref. 2]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Skaugen M., Abildgaard C.I., Nes I.F.
"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-67.
MEDLINE=91336724;PubMed=1872611;
Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F.
"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45.", Appl. Environ. Microbiol. 57:1829-1834(1991).

Gene structure

Gene id	Name	Description	Location
BACGene197		hypothetical protein ORF78	1..237
BACGene198		hypothetical protein ORF116	230..580
BACGene199		hypothetical protein ORF130 N-terminally truncated gene product;coding sequence interrupted by insertionsequence	complement(799..1200)
BACGene200		hypothetical protein ORF84 Putative, N-terminally truncated transposase;coding sequence interrupted byinsertion sequence	complement(1209..1460)
BACGene201	tnp1519L	putative IS1519L transposase	1536..2216
BACGene202	tnp1516delC	hypothetical protein N-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence.	2897..3352
BACGene203	tnp1516delN	putative protein C-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence.	3353..4168
BACGene204	lasY	hypothetical protein	complement(4573..5475)
BACGene205	lasX	putative Rgg-type transcriptional regulator essential for normal lactocin S production	complement(5546..6403)
BACGene206	lasA	pre-lactocin S	6586..6792
BACGene207	lasM	hypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification	6885..9662
BACGene208	lasN	hypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification	9677..10396
BACGene209	lasT	ABC transporter probable exporter of mature lactocin S; essential for lactocin S production	10399..12006
BACGene210	lasU	hypothetical protein	12003..12284
BACGene211	lasV	hypothetical protein	12297..12674
BACGene212	lasP	serine protease probably involved in pre-lactocin S processing	12671..13471
BACGene213	lasJ	hypothetical protein probably involved in lactocin S immunity	13491..13664
BACGene214	lasW	hypothetical protein	13702..14946
BACGene215	tnp1519R	putative IS1519R transposase	15068..15754

Protein sequence

........10 ........20 ........30 ........40
| | | |
STPVLASVAV SMELLPTASV LYSDVAGCFK YSAKHHC

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔37	37	Bacteriocin lactocin-S. Feature identifier = PRO_0000002765.
Modified residue	1		Pyruvic acid (Ser).
Modified residue	2		2,3-didehydrobutyrine.
Modified residue	7		D-alanine (Ser).
Modified residue	11		D-alanine (Ser).
Modified residue	19		D-alanine (Ser).
Cross-link	23↔28	6	Lanthionine (Ser-Cys) (Probable).
Cross-link	32↔37	6	Lanthionine (Ser-Cys) (Probable).

Composition

Hydrophobicity

Composition

Formula	C₁₇₃ H₂₇₃ N₄₃ O₅₂ S₃
Absent amino acids	INQRW
Common amino acids	S
Mass (Da)	3902.12
Net charge	+2
Isoelectric point	7.41
Basic residues	4
Acidic residues	2
Hydrophobic residues	15
Polar residues	13
Aliphatic residues	9
Tiny residues	12
Boman Index	-4.01
Hydropathy Index	0.58
Aliphatic Index	94.86
Instability Index	16.08 (stable)
Half Life	Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	3105 M^-1 cm^-1
Absorbance 280nm	86.25

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