Bacteriocin: lactocin-S

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Bacteriocin: lactocin-S

Accession: BAC057

Classification
Class: Lantibiotic
Genetics
Gene: lasA
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: P23826 Q48848 Q48858

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=95050447;PubMed=7961627;
Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F.
"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide.", J. Biol. Chem. 269:27183-27185(1994).
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Skaugen M., Abildgaard C.I., Nes I.F.
"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-67.
MEDLINE=91336724;PubMed=1872611;
Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F.
"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45.", Appl. Environ. Microbiol. 57:1829-1834(1991).
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Gene structure
genome of lactocin-S

Gene idNameDescriptionLocation
BACGene197hypothetical protein ORF781..237
BACGene198hypothetical protein ORF116230..580
BACGene199hypothetical protein ORF130 N-terminally truncated gene product;coding sequence interrupted by insertionsequencecomplement(799..1200)
BACGene200hypothetical protein ORF84 Putative, N-terminally truncated transposase;coding sequence interrupted byinsertion sequencecomplement(1209..1460)
BACGene201tnp1519Lputative IS1519L transposase1536..2216
BACGene202tnp1516delChypothetical protein N-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence.2897..3352
BACGene203tnp1516delNputative protein C-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence.3353..4168
BACGene204lasYhypothetical proteincomplement(4573..5475)
BACGene205lasXputative Rgg-type transcriptional regulator essential for normal lactocin S productioncomplement(5546..6403)
BACGene206lasApre-lactocin S6586..6792
BACGene207lasMhypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification6885..9662
BACGene208lasNhypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification9677..10396
BACGene209lasTABC transporter probable exporter of mature lactocin S; essential for lactocin S production10399..12006
BACGene210lasUhypothetical protein12003..12284
BACGene211lasVhypothetical protein12297..12674
BACGene212lasPserine protease probably involved in pre-lactocin S processing12671..13471
BACGene213lasJhypothetical protein probably involved in lactocin S immunity13491..13664
BACGene214lasWhypothetical protein13702..14946
BACGene215tnp1519Rputative IS1519R transposase15068..15754
tnp1519Ltnp1516delCtnp1516delNlasYlasXlasAlasMlasNlasTlasUlasVlasPlasJlasWtnp1519R
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 STPVLASVAV SMELLPTASV LYSDVAGCFK YSAKHHC

Wheel representation
wheel representation of lactocin-S
Protein sequence annotations
Features of lactocin-S

STSSS
FeaturePosition(s)LengthDescription
CHAIN1↔3737
Bacteriocin lactocin-S.
Feature identifier = PRO_0000002765.
Modified residue1Pyruvic acid (Ser).
Modified residue22,3-didehydrobutyrine.
Modified residue7D-alanine (Ser).
Modified residue11D-alanine (Ser).
Modified residue19D-alanine (Ser).
Cross-link23↔286Lanthionine (Ser-Cys) (Probable).
Cross-link32↔376Lanthionine (Ser-Cys) (Probable).
Composition
Hydrophobicity

Composition
Formula C173 H273 N43 O52 S3
Absent amino acids INQRW
Common amino acids S
Mass (Da) 3902.12
Net charge +2
Isoelectric point 7.41
Basic residues 4
Acidic residues 2
Hydrophobic residues 15
Polar residues 13
Aliphatic residues 9
Tiny residues 12
Boman Index -4.01
Hydropathy Index 0.58
Aliphatic Index 94.86
Instability Index 16.08 (stable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 3105 M-1 cm-1
Absorbance 280nm 86.25

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