Bacteriocin: lactocin-S
Accession: BAC057
Classification
Class: Lantibiotic
Genetics
Gene: lasA
Producer and target organisms
Producer Organism: Lactobacillus sakei L45 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsLeuconostoc - Carnobacteria - Lactobacilli - Pediococci - Lactococci - Listeria monocytogenes - Listeria innocua - Staphylococcus - Bacillus cereus - Clostridium spp
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=95050447;PubMed=7961627;
Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F.
"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide.", J. Biol. Chem. 269:27183-27185(1994).
MEDLINE=95050447;PubMed=7961627;
Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F.
"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide.", J. Biol. Chem. 269:27183-27185(1994).
[Ref. 2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Skaugen M., Abildgaard C.I., Nes I.F.
"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
Skaugen M., Abildgaard C.I., Nes I.F.
"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S.", Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-67.
MEDLINE=91336724;PubMed=1872611;
Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F.
"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45.", Appl. Environ. Microbiol. 57:1829-1834(1991).
MEDLINE=91336724;PubMed=1872611;
Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F.
"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45.", Appl. Environ. Microbiol. 57:1829-1834(1991).
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Gene structure
Gene id | Name | Description | Location |
BACGene197 | hypothetical protein ORF78 | 1..237 | |
BACGene198 | hypothetical protein ORF116 | 230..580 | |
BACGene199 | hypothetical protein ORF130 N-terminally truncated gene product;coding sequence interrupted by insertionsequence | complement(799..1200) | |
BACGene200 | hypothetical protein ORF84 Putative, N-terminally truncated transposase;coding sequence interrupted byinsertion sequence | complement(1209..1460) | |
BACGene201 | tnp1519L | putative IS1519L transposase | 1536..2216 |
BACGene202 | tnp1516delC | hypothetical protein N-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence. | 2897..3352 |
BACGene203 | tnp1516delN | putative protein C-terminal part of IS1516 transposase. Coding sequence probably interrupted bynonsense mutation in codon 152 of original sequence. | 3353..4168 |
BACGene204 | lasY | hypothetical protein | complement(4573..5475) |
BACGene205 | lasX | putative Rgg-type transcriptional regulator essential for normal lactocin S production | complement(5546..6403) |
BACGene206 | lasA | pre-lactocin S | 6586..6792 |
BACGene207 | lasM | hypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification | 6885..9662 |
BACGene208 | lasN | hypothetical protein essential for lactocin S production; probably involved in post-translationalpeptide modification | 9677..10396 |
BACGene209 | lasT | ABC transporter probable exporter of mature lactocin S; essential for lactocin S production | 10399..12006 |
BACGene210 | lasU | hypothetical protein | 12003..12284 |
BACGene211 | lasV | hypothetical protein | 12297..12674 |
BACGene212 | lasP | serine protease probably involved in pre-lactocin S processing | 12671..13471 |
BACGene213 | lasJ | hypothetical protein probably involved in lactocin S immunity | 13491..13664 |
BACGene214 | lasW | hypothetical protein | 13702..14946 |
BACGene215 | tnp1519R | putative IS1519R transposase | 15068..15754 |
Protein sequence
........10 ........20 ........30 ........40 | | | | STPVLASVAV SMELLPTASV LYSDVAGCFK YSAKHHC |
Wheel representation
Protein sequence annotations
Feature | Position(s) | Length | Description |
CHAIN | 1↔37 | 37 | Bacteriocin lactocin-S. Feature identifier = PRO_0000002765. |
Modified residue | 1 | Pyruvic acid (Ser). | |
Modified residue | 2 | 2,3-didehydrobutyrine. | |
Modified residue | 7 | D-alanine (Ser). | |
Modified residue | 11 | D-alanine (Ser). | |
Modified residue | 19 | D-alanine (Ser). | |
Cross-link | 23↔28 | 6 | Lanthionine (Ser-Cys) (Probable). |
Cross-link | 32↔37 | 6 | Lanthionine (Ser-Cys) (Probable). |
Composition
Hydrophobicity
Composition
Formula | C173
H273
N43
O52
S3 |
Absent amino acids | INQRW |
Common amino acids | S |
Mass (Da) | 3902.12 |
Net charge | +2 |
Isoelectric point | 7.41 |
Basic residues | 4 |
Acidic residues | 2 |
Hydrophobic residues | 15 |
Polar residues | 13 |
Aliphatic residues | 9 |
Tiny residues | 12 |
Boman Index | -4.01 |
Hydropathy Index | 0.58 |
Aliphatic Index | 94.86 |
Instability Index | 16.08 (stable) |
Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 3105 M-1 cm-1 |
Absorbance 280nm | 86.25 |
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