Bacteriocin: Nisin Z

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Bacteriocin: Nisin Z

Accession: BAC049

Classification

Class: Lantibiotic

Genetics

Gene: nisZ

Producer and target organisms

Producer Organism: Lactococcus lactis subsp (Streptococcus lactis), lactis [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsactive on Gram-positive bacteria: Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - clostridium

Description

Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.

Related Entries

Nisin A Nisin Q Nisin F nisin U

Uniprot and PDB links

UniProt Entry: P29559

PDB Entry1WCO resolved by NMR

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=NIZO 22186
MEDLINE=92037612;PubMed=1935953;DOI=10.1111/j.1432-1033.1991.tb16317.x
Mulders J.W.M., Boerrigter I.J., Rollema H.S., Siezen R.J., de Vos W.M.
"Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.", Eur. J. Biochem. 201:581-584(1991).

[Ref. 2]

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JCM 7638
DOI=10.2323/jgam.38.271
Araya T., Ishibashi N., Shimamura S.
"Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form of nisin.", J. Gen. Appl. Microbiol. 38:271-278(1992).

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=N8
MEDLINE=95352820;PubMed=7626780;
Immonen T., Ye S., Ra R., Qiao M., Paulin L., Saris P.E.J.
"The codon usage of the nisZ operon in Lactococcus lactis N8 suggests a non-lactococcal origin of the conjugative nisin-sucrose transposon.", DNA Seq. 5:203-218(1995).

Gene structure

Gene id	Name	Description	Location
BACGene177	nisin	NisZ protein antibiotic	239..412
BACGene178	nisB	NisB protein	520..3501
BACGene179	nisT	NisT protein	3512..5314
BACGene180	nisC	NisC protein	5295..6551
BACGene181	nisI	NisI protein	6548..7285
BACGene182	nisP	NisP protein	7287..7610

Protein sequence

........10 ........20 ........30 ........40
| | | |
ITSISLCTPG CKTGALMGCN MKTATCNCSI HVSK

Wheel representation

3D Structure

Protein sequence annotations

Feature	Position(s)	Length	Description
PEPTIDE	1↔34	34	Lantibiotic nisin-Z. Feature identifier = PRO_0000017125.
Modified residue	2		2,3-didehydrobutyrine.
Modified residue	5		2,3-didehydroalanine (Ser).
Modified residue	33		2,3-didehydroalanine (Ser).
Cross-link	3↔7	5	Lanthionine (Ser-Cys).
Cross-link	8↔11	4	Beta-methyllanthionine (Thr-Cys).
Cross-link	13↔19	7	Beta-methyllanthionine (Thr-Cys).
Cross-link	23↔26	4	Beta-methyllanthionine (Thr-Cys).
Cross-link	25↔28	4	Beta-methyllanthionine (Thr-Cys).
VARIANT	27		N -> H (Nisin A).
VARIANT	30		I -> V (Nisin F).
HELIX	9↔12	4
TURN	15↔17	3

Composition

Hydrophobicity

Composition

Formula	C₁₄₁ H₂₄₅ N₄₁ O₄₆ S₇
Absent amino acids	DEFQRWY
Common amino acids	CT
Mass (Da)	3493.74
Net charge	+4
Isoelectric point	8.51
Basic residues	4
Acidic residues	0
Hydrophobic residues	8
Polar residues	19
Aliphatic residues	6
Tiny residues	9
Boman Index	-14.86
Hydropathy Index	0.41
Aliphatic Index	71.76
Instability Index	17.08 (stable)
Half Life	Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour
Extinction Coefficient	250 M^-1 cm^-1
Absorbance 280nm	7.58

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