Bacteriocin: Nisin Z
Accession: BAC049
Classification
Class: Lantibiotic
Genetics
Gene: nisZ
Producer and target organisms
Producer Organism: Lactococcus lactis subsp (Streptococcus lactis), lactis [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Streptococcaceae
Lactococcus
Target organismsactive on Gram-positive bacteria: Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - clostridium
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: P29559
PDB Entry1WCO resolved by NMR
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=NIZO 22186
MEDLINE=92037612;PubMed=1935953;DOI=10.1111/j.1432-1033.1991.tb16317.x
Mulders J.W.M., Boerrigter I.J., Rollema H.S., Siezen R.J., de Vos W.M.
"Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.", Eur. J. Biochem. 201:581-584(1991).
MEDLINE=92037612;PubMed=1935953;DOI=10.1111/j.1432-1033.1991.tb16317.x
Mulders J.W.M., Boerrigter I.J., Rollema H.S., Siezen R.J., de Vos W.M.
"Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.", Eur. J. Biochem. 201:581-584(1991).
[Ref. 2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JCM 7638
DOI=10.2323/jgam.38.271
Araya T., Ishibashi N., Shimamura S.
"Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form of nisin.", J. Gen. Appl. Microbiol. 38:271-278(1992).
DOI=10.2323/jgam.38.271
Araya T., Ishibashi N., Shimamura S.
"Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form of nisin.", J. Gen. Appl. Microbiol. 38:271-278(1992).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=N8
MEDLINE=95352820;PubMed=7626780;
Immonen T., Ye S., Ra R., Qiao M., Paulin L., Saris P.E.J.
"The codon usage of the nisZ operon in Lactococcus lactis N8 suggests a non-lactococcal origin of the conjugative nisin-sucrose transposon.", DNA Seq. 5:203-218(1995).
MEDLINE=95352820;PubMed=7626780;
Immonen T., Ye S., Ra R., Qiao M., Paulin L., Saris P.E.J.
"The codon usage of the nisZ operon in Lactococcus lactis N8 suggests a non-lactococcal origin of the conjugative nisin-sucrose transposon.", DNA Seq. 5:203-218(1995).
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Gene structure
Gene id | Name | Description | Location |
BACGene177 | nisin | NisZ protein antibiotic | 239..412 |
BACGene178 | nisB | NisB protein | 520..3501 |
BACGene179 | nisT | NisT protein | 3512..5314 |
BACGene180 | nisC | NisC protein | 5295..6551 |
BACGene181 | nisI | NisI protein | 6548..7285 |
BACGene182 | nisP | NisP protein | 7287..7610 |
Protein sequence
........10 ........20 ........30 ........40 | | | | ITSISLCTPG CKTGALMGCN MKTATCNCSI HVSK |
Wheel representation
3D Structure
Protein sequence annotations
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔34 | 34 | Lantibiotic nisin-Z. Feature identifier = PRO_0000017125.
|
Modified residue | 2 | 2,3-didehydrobutyrine. | |
Modified residue | 5 | 2,3-didehydroalanine (Ser). | |
Modified residue | 33 | 2,3-didehydroalanine (Ser). | |
Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys). |
Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys). |
Cross-link | 13↔19 | 7 | Beta-methyllanthionine (Thr-Cys). |
Cross-link | 23↔26 | 4 | Beta-methyllanthionine (Thr-Cys). |
Cross-link | 25↔28 | 4 | Beta-methyllanthionine (Thr-Cys). |
VARIANT | 27 | N -> H (Nisin A). | |
VARIANT | 30 | I -> V (Nisin F). | |
HELIX | 9↔12 | 4 | |
TURN | 15↔17 | 3 |
Composition
Hydrophobicity
Composition
Formula | C141
H245
N41
O46
S7 |
Absent amino acids | DEFQRWY |
Common amino acids | CT |
Mass (Da) | 3493.74 |
Net charge | +4 |
Isoelectric point | 8.51 |
Basic residues | 4 |
Acidic residues | 0 |
Hydrophobic residues | 8 |
Polar residues | 19 |
Aliphatic residues | 6 |
Tiny residues | 9 |
Boman Index | -14.86 |
Hydropathy Index | 0.41 |
Aliphatic Index | 71.76 |
Instability Index | 17.08 (stable) |
Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
Extinction Coefficient | 250 M-1 cm-1 |
Absorbance 280nm | 7.58 |
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