Bacteriocin: Nisin Z

Default view

Bacteriocin: Nisin Z

Accession: BAC049

Classification
Class: Lantibiotic
Genetics
Gene: nisZ
Producer and target organisms

Producer Organism: Lactococcus lactis subsp (Streptococcus lactis), lactis [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsactive on Gram-positive bacteria: Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - clostridium

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: P29559

PDB Entry1WCO resolved by NMR

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=NIZO 22186
MEDLINE=92037612;PubMed=1935953;DOI=10.1111/j.1432-1033.1991.tb16317.x
Mulders J.W.M., Boerrigter I.J., Rollema H.S., Siezen R.J., de Vos W.M.
"Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.", Eur. J. Biochem. 201:581-584(1991).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JCM 7638
DOI=10.2323/jgam.38.271
Araya T., Ishibashi N., Shimamura S.
"Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form of nisin.", J. Gen. Appl. Microbiol. 38:271-278(1992).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=N8
MEDLINE=95352820;PubMed=7626780;
Immonen T., Ye S., Ra R., Qiao M., Paulin L., Saris P.E.J.
"The codon usage of the nisZ operon in Lactococcus lactis N8 suggests a non-lactococcal origin of the conjugative nisin-sucrose transposon.", DNA Seq. 5:203-218(1995).
Loading...
End Note
Reference Manager
BibTeX
Gene structure
genome of Nisin Z

Gene idNameDescriptionLocation
BACGene177nisinNisZ protein antibiotic239..412
BACGene178nisBNisB protein520..3501
BACGene179nisTNisT protein3512..5314
BACGene180nisCNisC protein5295..6551
BACGene181nisINisI protein6548..7285
BACGene182nisPNisP protein7287..7610
nisinnisBnisTnisCnisInisP
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 ITSISLCTPG CKTGALMGCN MKTATCNCSI HVSK

Wheel representation
wheel representation of Nisin Z
3D Structure
Protein sequence annotations
Features of Nisin Z

TSS
FeaturePosition(s)LengthDescription
PEPTIDE1↔3434
Lantibiotic nisin-Z.
Feature identifier = PRO_0000017125.
Modified residue22,3-didehydrobutyrine.
Modified residue52,3-didehydroalanine (Ser).
Modified residue332,3-didehydroalanine (Ser).
Cross-link3↔75Lanthionine (Ser-Cys).
Cross-link8↔114Beta-methyllanthionine (Thr-Cys).
Cross-link13↔197Beta-methyllanthionine (Thr-Cys).
Cross-link23↔264Beta-methyllanthionine (Thr-Cys).
Cross-link25↔284Beta-methyllanthionine (Thr-Cys).
VARIANT27N -> H (Nisin A).
VARIANT30I -> V (Nisin F).
HELIX9↔124
TURN15↔173
Composition
Hydrophobicity

Composition
Formula C141 H245 N41 O46 S7
Absent amino acids DEFQRWY
Common amino acids CT
Mass (Da) 3493.74
Net charge +4
Isoelectric point 8.51
Basic residues 4
Acidic residues 0
Hydrophobic residues 8
Polar residues 19
Aliphatic residues 6
Tiny residues 9
Boman Index -14.86
Hydropathy Index 0.41
Aliphatic Index 71.76
Instability Index 17.08 (stable)
Half Life Mammalian : 20 hour
Yeast : 30 min
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 7.58

Users comments for Nisin Z

No comments found

Login or register to submit a comment for Nisin Z.