Bacteriocin: Pep5
Accession: BAC048
Classification
Class: Lantibiotic
Genetics
Gene: pepA
Producer and target organisms
Producer Organism: Staphylococcus epidermidis [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus
Target organismsactive on Gram-positive bacteria
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
After proteolysis of the propeptide, the N-terminal 2,3- didehydrobutyrine hydrolyzes to 2-oxobutanoic acid, possibly spontaneously.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
After proteolysis of the propeptide, the N-terminal 2,3- didehydrobutyrine hydrolyzes to 2-oxobutanoic acid, possibly spontaneously.
Uniprot and PDB links
UniProt Entry: P19578
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=5
MEDLINE=89350399;PubMed=2764678;
Kaletta C., Entian K.-D., Kellner R., Jung G., Reis M., Sahl H.-G.
"Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence.", Arch. Microbiol. 152:16-19(1989).
MEDLINE=89350399;PubMed=2764678;
Kaletta C., Entian K.-D., Kellner R., Jung G., Reis M., Sahl H.-G.
"Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence.", Arch. Microbiol. 152:16-19(1989).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=5
MEDLINE=96035882;PubMed=7556197;
Meyer C., Bierbaum G., Heidrich C., Reis M., Suling J., Iglesias-Wind M., Kempter C., Molitor E., Sahl H.-G.
"Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. Evidence for a role of PepC in thioether formation.", Eur. J. Biochem. 232:478-489(1995).
MEDLINE=96035882;PubMed=7556197;
Meyer C., Bierbaum G., Heidrich C., Reis M., Suling J., Iglesias-Wind M., Kempter C., Molitor E., Sahl H.-G.
"Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. Evidence for a role of PepC in thioether formation.", Eur. J. Biochem. 232:478-489(1995).
BIOSYNTHESIS.
MEDLINE=91071193;PubMed=2253617;
Weil H.-P., Beck-Sickinger A.G., Metzger J., Stevanovic S., Jung G., Josten M.
"Biosynthesis of the lantibiotic Pep5. Isolation and characterization of a prepeptide containing dehydroamino acids.", Eur. J. Biochem. 194:217-223(1990).
MEDLINE=91071193;PubMed=2253617;
Weil H.-P., Beck-Sickinger A.G., Metzger J., Stevanovic S., Jung G., Josten M.
"Biosynthesis of the lantibiotic Pep5. Isolation and characterization of a prepeptide containing dehydroamino acids.", Eur. J. Biochem. 194:217-223(1990).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene171 | pepT | PepT | complement(88..1803) |
| BACGene172 | pepI | PepI | 2028..2237 |
| BACGene173 | pepA | PepA | 2322..2504 |
| BACGene174 | pepP | PepP | 2566..3423 |
| BACGene175 | pepB | PepB | 3410..6313 |
| BACGene176 | pepC | PepC | 6310..7506 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | TAGPAIRASV KQCQKTLKAT RLFTVSCKGK NGCK |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔34 | 34 | Lantibiotic Pep5. Feature identifier = PRO_0000017141. |
| Modified residue | 1 | 2-oxobutanoic acid (Probable). | |
| Modified residue | 16 | 2,3-didehydrobutyrine (Probable). | |
| Modified residue | 20 | 2,3-didehydrobutyrine (Probable). | |
| Cross-link | 9↔13 | 5 | Lanthionine (Ser-Cys) (Probable). |
| Cross-link | 24↔27 | 4 | Beta-methyllanthionine (Thr-Cys)(Probable). |
| Cross-link | 26↔33 | 8 | Lanthionine (Ser-Cys) (Probable). |
Composition
Hydrophobicity
Composition
| Formula | C153
H269
N49
O44
S3 |
| Absent amino acids | DEHMWY |
| Common amino acids | K |
| Mass (Da) | 3613.87 |
| Net charge | +8 |
| Isoelectric point | 11.08 |
| Basic residues | 8 |
| Acidic residues | 0 |
| Hydrophobic residues | 10 |
| Polar residues | 13 |
| Aliphatic residues | 5 |
| Tiny residues | 9 |
| Boman Index | -58.22 |
| Hydropathy Index | -0.36 |
| Aliphatic Index | 63.24 |
| Instability Index | 14.73 (stable) |
| Half Life |
Mammalian : 7.2 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 125 M-1 cm-1 |
| Absorbance 280nm | 3.79 |
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