Bacteriocin: Nisin A
Accession: BAC047
Classification
Class: Lantibiotic
Genetics
Gene: spaN; Synonyms=nisA
Producer and target organisms
Producer Organism: Lactococcus lactis subsp (Streptococcus lactis), lactis [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismsactive on Gram-positive bacteria: Enterococcus - Lactobacillus - Lactococcus - Leuconostoc - Listeria - clostridium
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Uniprot and PDB links
UniProt Entry: P13068
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11454 / DSM 20729 / NCDO 496
MEDLINE=89034093;PubMed=3141403;
Buchman G.W., Banerjee S., Hansen J.N.
"Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.", J. Biol. Chem. 263:16260-16266(1988).
MEDLINE=89034093;PubMed=3141403;
Buchman G.W., Banerjee S., Hansen J.N.
"Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic.", J. Biol. Chem. 263:16260-16266(1988).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=ATCC 11454 / DSM 20729 / NCDO 496
MEDLINE=91282469;PubMed=1905517;
Steen M.T., Chung Y.J., Hansen J.N.
"Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.", Appl. Environ. Microbiol. 57:1181-1188(1991).
MEDLINE=91282469;PubMed=1905517;
Steen M.T., Chung Y.J., Hansen J.N.
"Characterization of the nisin gene as part of a polycistronic operon in the chromosome of Lactococcus lactis ATCC 11454.", Appl. Environ. Microbiol. 57:1181-1188(1991).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=6F3
MEDLINE=89155467;PubMed=2493449;
Kaletta C., Entian K.-D.
"Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product.", J. Bacteriol. 171:1597-1601(1989).
MEDLINE=89155467;PubMed=2493449;
Kaletta C., Entian K.-D.
"Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product.", J. Bacteriol. 171:1597-1601(1989).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=6F3
MEDLINE=93128945;PubMed=1482192;
Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D.
"Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB protein.", Appl. Environ. Microbiol. 58:3730-3743(1992).
MEDLINE=93128945;PubMed=1482192;
Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D.
"Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB protein.", Appl. Environ. Microbiol. 58:3730-3743(1992).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=NIZO R5
MEDLINE=93373937;PubMed=7689965;
Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.
"Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity.", Eur. J. Biochem. 216:281-291(1993).
MEDLINE=93373937;PubMed=7689965;
Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.
"Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity.", Eur. J. Biochem. 216:281-291(1993).
PROTEIN SEQUENCE OF 24-57.
MEDLINE=72072901;PubMed=5131162;
Gross E., Morell J.L.
"The structure of nisin.", J. Am. Chem. Soc. 93:4634-4635(1971).
MEDLINE=72072901;PubMed=5131162;
Gross E., Morell J.L.
"The structure of nisin.", J. Am. Chem. Soc. 93:4634-4635(1971).
STRUCTURE BY NMR.
MEDLINE=92111494;PubMed=1765078;
van de Ven F.J., van den Hooven H.W., Konings R.N.H., Hilbers C.W.
"NMR studies of lantibiotics. The structure of nisin in aqueous solution.", Eur. J. Biochem. 202:1181-1188(1991).
MEDLINE=92111494;PubMed=1765078;
van de Ven F.J., van den Hooven H.W., Konings R.N.H., Hilbers C.W.
"NMR studies of lantibiotics. The structure of nisin in aqueous solution.", Eur. J. Biochem. 202:1181-1188(1991).
STRUCTURE BY NMR.
MEDLINE=92246867;PubMed=1575686;
Lian L.-Y., Chan W.C., Morley S.D., Roberts G.C.K., Bycroft B.W., Jackson D.
"Solution structures of nisin A and its two major degradation products determined by NMR.", Biochem. J. 283:413-420(1992).
MEDLINE=92246867;PubMed=1575686;
Lian L.-Y., Chan W.C., Morley S.D., Roberts G.C.K., Bycroft B.W., Jackson D.
"Solution structures of nisin A and its two major degradation products determined by NMR.", Biochem. J. 283:413-420(1992).
STRUCTURE BY NMR.
MEDLINE=93202265;PubMed=8454055;DOI=10.1016/0014-5793(93)80065-3
van den Hooven H.W., Fogolari F., Rollema H.S., Konings R.N.H., Hilbers C.W., van de Ven F.J.
"NMR and circular dichroism studies of the lantibiotic nisin in non- aqueous environments.", FEBS Lett. 319:189-194(1993).
MEDLINE=93202265;PubMed=8454055;DOI=10.1016/0014-5793(93)80065-3
van den Hooven H.W., Fogolari F., Rollema H.S., Konings R.N.H., Hilbers C.W., van de Ven F.J.
"NMR and circular dichroism studies of the lantibiotic nisin in non- aqueous environments.", FEBS Lett. 319:189-194(1993).
STRUCTURE BY NMR.
MEDLINE=93120109;PubMed=8418850;
Sailer M., Helms G.L., Henkel T., Niemczura W.P., Stiles M.E., Vederas J.C.
"15N- and 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.", Biochemistry 32:310-318(1993).
MEDLINE=93120109;PubMed=8418850;
Sailer M., Helms G.L., Henkel T., Niemczura W.P., Stiles M.E., Vederas J.C.
"15N- and 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.", Biochemistry 32:310-318(1993).
Loading...
Gene structure
| Gene id | Name | Description | Location |
| BACGene166 | nisX | transposase | 1..329 |
| BACGene167 | nisA | Nisin-prepeptide | 1159..1332 |
| BACGene168 | nisB | biosynthetic gene | 1439..4420 |
| BACGene169 | nisT | transport of Nisin | 4431..6233 |
| BACGene170 | nisC | biosynthetic gene | 6214..7470 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | ITSISLCTPG CKTGALMGCN MKTATCHCSI HVSK |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔34 | 34 | Lantibiotic nisin-A. Feature identifier = PRO_0000017123. |
| Modified residue | 2 | (Z)-2,3-didehydrobutyrine. | |
| Modified residue | 5 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 33 | 2,3-didehydroalanine (Ser). | |
| Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 13↔19 | 7 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 23↔26 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 25↔28 | 4 | Beta-methyllanthionine (Thr-Cys). |
Composition
Hydrophobicity
Composition
| Formula | C143
H246
N42
O45
S7 |
| Absent amino acids | DEFQRWY |
| Common amino acids | CT |
| Mass (Da) | 3516.78 |
| Net charge | +5 |
| Isoelectric point | 8.52 |
| Basic residues | 5 |
| Acidic residues | 0 |
| Hydrophobic residues | 8 |
| Polar residues | 18 |
| Aliphatic residues | 6 |
| Tiny residues | 9 |
| Boman Index | -12.88 |
| Hydropathy Index | 0.41 |
| Aliphatic Index | 71.76 |
| Instability Index | 27.52 (stable) |
| Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 250 M-1 cm-1 |
| Absorbance 280nm | 7.58 |
Users comments for Nisin A
No comments found