Bacteriocin: epilancin K7
Accession: BAC043
Classification
Class: Lantibiotic
Genetics
Gene: elkA
Producer and target organisms
Producer Organism: Staphylococcus epidermidis [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus
Target organismsUnavailable data
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: Q57312
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=K7
MEDLINE=95331297;PubMed=7607233;
De Kamp M., den Hooven H.W., Konings R.N., Bierbaum G., Sahl H.G., Kuipers O.P., Siezen R.J., de Vos W.M., Hilbers C.W., de Ven F.J.
"Elucidation of the primary structure of the lantibiotic epilancin K7 from Staphylococcus epidermidis K7. Cloning and characterisation of the epilancin-K7-encoding gene and NMR analysis of mature epilancin K7.", Eur. J. Biochem. 230:587-600(1995).
MEDLINE=95331297;PubMed=7607233;
De Kamp M., den Hooven H.W., Konings R.N., Bierbaum G., Sahl H.G., Kuipers O.P., Siezen R.J., de Vos W.M., Hilbers C.W., de Ven F.J.
"Elucidation of the primary structure of the lantibiotic epilancin K7 from Staphylococcus epidermidis K7. Cloning and characterisation of the epilancin-K7-encoding gene and NMR analysis of mature epilancin K7.", Eur. J. Biochem. 230:587-600(1995).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=K7
van de Kamp M.
"", Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
van de Kamp M.
"", Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
[Ref. 3]
NUCLEOTIDE SEQUENCE, STRAIN=K7
Van de Kamp M.
"", Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
Van de Kamp M.
"", Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene150 | elkT | translocator | complement(1..285) |
| BACGene151 | elkA | lantibiotic epilancin K7 pre-epilancin K7 | 466..633 |
| BACGene152 | elkP | leader peptidase | 721..1110 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | SASVLKTSIK VSKKYCKGVT LTCGCNITGG K |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔31 | 31 | Lantibiotic epilancin. Feature identifier = PRO_0000275928. |
| Modified residue | 1 | Lactic acid. | |
| Modified residue | 3 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 7 | 2,3-didehydrobutyrine. | |
| Modified residue | 8 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 28 | 2,3-didehydrobutyrine. | |
| Cross-link | 12↔16 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 20↔23 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 22↔25 | 4 | Beta-methyllanthionine (Thr-Cys). |
Composition
Hydrophobicity
Composition
| Formula | C136
H240
N38
O42
S3 |
| Absent amino acids | DEFHMPQRW |
| Common amino acids | K |
| Mass (Da) | 3194.32 |
| Net charge | +6 |
| Isoelectric point | 10.3 |
| Basic residues | 6 |
| Acidic residues | 0 |
| Hydrophobic residues | 8 |
| Polar residues | 17 |
| Aliphatic residues | 7 |
| Tiny residues | 9 |
| Boman Index | -22.75 |
| Hydropathy Index | 0.09 |
| Aliphatic Index | 81.61 |
| Instability Index | 8.66 (stable) |
| Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 1615 M-1 cm-1 |
| Absorbance 280nm | 53.83 |
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