Bacteriocin: epilancin K7

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Bacteriocin: epilancin K7

Accession: BAC043

Classification
Class: Lantibiotic
Genetics
Gene: elkA
Producer and target organisms

Producer Organism: Staphylococcus epidermidis [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesStaphylococcus

Target organismsUnavailable data

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: Q57312

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=K7
MEDLINE=95331297;PubMed=7607233;
De Kamp M., den Hooven H.W., Konings R.N., Bierbaum G., Sahl H.G., Kuipers O.P., Siezen R.J., de Vos W.M., Hilbers C.W., de Ven F.J.
"Elucidation of the primary structure of the lantibiotic epilancin K7 from Staphylococcus epidermidis K7. Cloning and characterisation of the epilancin-K7-encoding gene and NMR analysis of mature epilancin K7.", Eur. J. Biochem. 230:587-600(1995).
NUCLEOTIDE SEQUENCE, STRAIN=K7
van de Kamp M.
"", Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=K7
Van de Kamp M.
"", Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
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Gene structure
genome of epilancin K7

Gene idNameDescriptionLocation
BACGene150elkTtranslocatorcomplement(1..285)
BACGene151elkAlantibiotic epilancin K7 pre-epilancin K7466..633
BACGene152elkPleader peptidase721..1110
elkTelkAelkP
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 SASVLKTSIK VSKKYCKGVT LTCGCNITGG K

Wheel representation
wheel representation of epilancin K7
Protein sequence annotations
Features of epilancin K7

SSTST
FeaturePosition(s)LengthDescription
PEPTIDE1↔3131
Lantibiotic epilancin.
Feature identifier = PRO_0000275928.
Modified residue1Lactic acid.
Modified residue32,3-didehydroalanine (Ser).
Modified residue72,3-didehydrobutyrine.
Modified residue82,3-didehydroalanine (Ser).
Modified residue282,3-didehydrobutyrine.
Cross-link12↔165Lanthionine (Ser-Cys).
Cross-link20↔234Beta-methyllanthionine (Thr-Cys).
Cross-link22↔254Beta-methyllanthionine (Thr-Cys).
Composition
Hydrophobicity

Composition
Formula C136 H240 N38 O42 S3
Absent amino acids DEFHMPQRW
Common amino acids K
Mass (Da) 3194.32
Net charge +6
Isoelectric point 10.3
Basic residues 6
Acidic residues 0
Hydrophobic residues 8
Polar residues 17
Aliphatic residues 7
Tiny residues 9
Boman Index -22.75
Hydropathy Index 0.09
Aliphatic Index 81.61
Instability Index 8.66 (stable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 1615 M-1 cm-1
Absorbance 280nm 53.83

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