Bacteriocin: Epicidin 280
Accession: BAC040
Classification
Class: Lantibiotic
Genetics
Gene: eciA
Producer and target organisms
Producer Organism: Staphylococcus epidermidis [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus
Target organismsUnavailable data
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic) Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic) Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: O54220
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=BN 280
MEDLINE=98394975;PubMed=9726851;
Heidrich C., Pag U., Josten M., Metzger J., Jack R.W., Bierbaum G., Jung G., Sahl H.G.
"Isolation, characterization and sequence of the novel lantibiotic epicidin 280 and its biosynthetic gene cluster.", Appl. Environ. Microbiol. 64:3140-3146(1998).
MEDLINE=98394975;PubMed=9726851;
Heidrich C., Pag U., Josten M., Metzger J., Jack R.W., Bierbaum G., Jung G., Sahl H.G.
"Isolation, characterization and sequence of the novel lantibiotic epicidin 280 and its biosynthetic gene cluster.", Appl. Environ. Microbiol. 64:3140-3146(1998).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene143 | tnp1 | transposase | complement(1..30) |
| BACGene144 | eciO | oxidoreductase | 874..1617 |
| BACGene145 | eciI | EciI protein producer-self-protection | 1690..1878 |
| BACGene146 | eciA | epicidin 280 antimicrobial | 1940..2110 |
| BACGene147 | eciP | protease subtilisin-like leader peptidase | 2194..3096 |
| BACGene148 | eciB | EciB protein | 3083..6013 |
| BACGene149 | eciC | EciC protein | 5998..7191 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | SLGPAIKATR QVCPKATRFV TVSCKKSDCQ |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔30 | 30 | epicidin 280. Feature identifier = PRO_5000147185. |
Composition
Hydrophobicity
Composition
| Formula | C137
H236
N42
O41
S3 |
| Absent amino acids | EHMNWY |
| Common amino acids | K |
| Mass (Da) | 3242.31 |
| Net charge | +5 |
| Isoelectric point | 10.35 |
| Basic residues | 6 |
| Acidic residues | 1 |
| Hydrophobic residues | 9 |
| Polar residues | 10 |
| Aliphatic residues | 5 |
| Tiny residues | 7 |
| Boman Index | -54.6 |
| Hydropathy Index | -0.22 |
| Aliphatic Index | 65 |
| Instability Index | 29.11 (stable) |
| Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 125 M-1 cm-1 |
| Absorbance 280nm | 4.31 |
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