Bacteriocin: Nukacin ISK-1
Accession: BAC037
Classification
Class: Lantibiotic
Genetics
Gene: nukA
Producer and target organisms
Producer Organism: Staphylococcus warneri [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus
Target organismsBacillus subtilis JCM 1465 (MIC= 25 �M) - Lactobacillus sakei JCM 1157 (MIC= 0.39 �M) - Micrococcus luteus DSM 1790 (MIC= 0.78 �M) - Staphylococcus simulans 22 (MIC= 0.78 �M) - Staphylococcus aureus ATCC 12600 (MIC= 50 �M) - Streptococcus bovis JCM 5802 (MIC= 1.56 �M) - Pediococcus pentosaceus JCM 5890 (MIC= 0.78 �M)
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Producer strain:
Staphylococcus simulans produces Nukacin 3299 (formerly designated simulancin 3299), a bacteriocin that is identical to nukacin ISK-1 [view ref].
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Producer strain:
Staphylococcus simulans produces Nukacin 3299 (formerly designated simulancin 3299), a bacteriocin that is identical to nukacin ISK-1 [view ref].
Uniprot and PDB links
UniProt Entry: Q9KWM4
PDB Entry5Z5Q resolved by NMR 5Z5R resolved by NMR
[Ref. 1]
NUCLEOTIDE SEQUENCE.
Kimura H., Matsusaki H., Sashihara T., Sonomoto K., Ishizaki A.
"Purification and partial identifiaction of bacteriocin ISK-1, a new lantibiotic produced by Pediococcus sp. ISK-1.", Biosci. Biotechnol. Biochem. 62:2341-2345(1998).
Kimura H., Matsusaki H., Sashihara T., Sonomoto K., Ishizaki A.
"Purification and partial identifiaction of bacteriocin ISK-1, a new lantibiotic produced by Pediococcus sp. ISK-1.", Biosci. Biotechnol. Biochem. 62:2341-2345(1998).
[Ref. 2]
NUCLEOTIDE SEQUENCE.
Sashihara T., Kimura H., Higuchi T., Matsusaki H., Sonomoto K., Ishizaki A.
"", Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Sashihara T., Kimura H., Higuchi T., Matsusaki H., Sonomoto K., Ishizaki A.
"", Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
PubMed=15322349;DOI=10.1271/bbb.68.1663
Aso Y., Sashihara T., Nagao J., Kanemasa Y., Koga H., Hashimoto T., Higuchi T., Adachi A., Nomiyama H., Ishizaki A., Nakayama J., Sonomoto K.
"Characterization of a Gene Cluster of Staphylococcus warneri ISK-1 Encoding the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", Biosci. Biotechnol. Biochem. 68:1663-1671(2004).
PubMed=15322349;DOI=10.1271/bbb.68.1663
Aso Y., Sashihara T., Nagao J., Kanemasa Y., Koga H., Hashimoto T., Higuchi T., Adachi A., Nomiyama H., Ishizaki A., Nakayama J., Sonomoto K.
"Characterization of a Gene Cluster of Staphylococcus warneri ISK-1 Encoding the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", Biosci. Biotechnol. Biochem. 68:1663-1671(2004).
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
MEDLINE=21036904;PubMed=11193411;DOI=10.1271/bbb.64.2420
Sashihara T., Kimura H., Higuchi T., Adachi A., Matsusaki H., Sonomoto K., Ishizaki A.
"A Novel Lantibiotic, Nukacin ISK-1, of Staphylococcus warneri ISK-1: Cloning of the Structural Gene and Identification of the Structure.", Biosci. Biotechnol. Biochem. 64:2420-2428(2000).
MEDLINE=21036904;PubMed=11193411;DOI=10.1271/bbb.64.2420
Sashihara T., Kimura H., Higuchi T., Adachi A., Matsusaki H., Sonomoto K., Ishizaki A.
"A Novel Lantibiotic, Nukacin ISK-1, of Staphylococcus warneri ISK-1: Cloning of the Structural Gene and Identification of the Structure.", Biosci. Biotechnol. Biochem. 64:2420-2428(2000).
NUCLEOTIDE SEQUENCE.
PubMed=16233732;
Aso Y., Nagao J., Koga H., Okuda K., Kanemasa Y., Sashihara T., Nakayama J., Sonomoto K.
"Heterologous Expression and Functional Analysis of the Gene Cluster for Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", J. Biosci. Bioeng. 98:429-436(2004).
PubMed=16233732;
Aso Y., Nagao J., Koga H., Okuda K., Kanemasa Y., Sashihara T., Nakayama J., Sonomoto K.
"Heterologous Expression and Functional Analysis of the Gene Cluster for Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", J. Biosci. Bioeng. 98:429-436(2004).
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
PubMed=15737403;DOI=10.1016/j.plasmid.2004.08.003
Aso Y., Koga H., Sashihara T., Nagao J., Kanemasa Y., Nakayama J., Sonomoto K.
"Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1.", Plasmid 53:164-178(2005).
PubMed=15737403;DOI=10.1016/j.plasmid.2004.08.003
Aso Y., Koga H., Sashihara T., Nagao J., Kanemasa Y., Nakayama J., Sonomoto K.
"Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1.", Plasmid 53:164-178(2005).
FUNCTION AND MECHANISM OF ACTION
PubMed=25229869;DOI=10.1080/09168451.2014.918485
Roy U., Islam M.R., Nagao J., Iida H., Mahin A., Li M., Zendo T., Nakayama J., Sonomoto K.
"Bactericidal activity of nukacin ISK-1: an alternative mode of action.", Biosci Biotechnol Biochem 2014, 78 (7):1270-3.
PubMed=25229869;DOI=10.1080/09168451.2014.918485
Roy U., Islam M.R., Nagao J., Iida H., Mahin A., Li M., Zendo T., Nakayama J., Sonomoto K.
"Bactericidal activity of nukacin ISK-1: an alternative mode of action.", Biosci Biotechnol Biochem 2014, 78 (7):1270-3.
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Protein sequence
| ........10 ........20 ........30 | | | KKKSGVIPTV SHDCHMNSFQ FVFTCCS |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔27 | 27 | Lantibiotic nukacin. Feature identifier = PRO_0000307095.
|
| Modified residue | 24 | 2,3-didehydrobutyrine (Probable). | |
| Cross-link | 9↔14 | 6 | Beta-methyllanthionine (Thr-Cys)(Probable). |
| Cross-link | 11↔25 | 15 | Lanthionine (Ser-Cys) (Probable). |
| Cross-link | 18↔26 | 9 | Lanthionine (Ser-Cys) (Probable). |
Composition
Hydrophobicity
Composition
| Formula | C132
H204
N36
O38
S4 |
| Absent amino acids | AELRWY |
| Common amino acids | S |
| Mass (Da) | 3049.96 |
| Net charge | +4 |
| Isoelectric point | 8.53 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 7 |
| Polar residues | 11 |
| Aliphatic residues | 4 |
| Tiny residues | 5 |
| Boman Index | -32.5 |
| Hydropathy Index | -0.01 |
| Aliphatic Index | 46.67 |
| Instability Index | 17.84 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 125 M-1 cm-1 |
| Absorbance 280nm | 4.81 |
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