Bacteriocin: Nukacin ISK-1

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Bacteriocin: Nukacin ISK-1

Accession: BAC037

Classification
Class: Lantibiotic
Genetics
Gene: nukA
Producer and target organisms

Producer Organism: Staphylococcus warneri [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesStaphylococcus

Target organismsBacillus subtilis JCM 1465 (MIC= 25 �M) - Lactobacillus sakei JCM 1157 (MIC= 0.39 �M) - Micrococcus luteus DSM 1790 (MIC= 0.78 �M) - Staphylococcus simulans 22 (MIC= 0.78 �M) - Staphylococcus aureus ATCC 12600 (MIC= 50 �M) - Streptococcus bovis JCM 5802 (MIC= 1.56 �M) - Pediococcus pentosaceus JCM 5890 (MIC= 0.78 �M)

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.

Producer strain:
Staphylococcus simulans produces Nukacin 3299 (formerly designated simulancin 3299), a bacteriocin that is identical to nukacin ISK-1 [view ref].
Uniprot and PDB links
NUCLEOTIDE SEQUENCE.
Kimura H., Matsusaki H., Sashihara T., Sonomoto K., Ishizaki A.
"Purification and partial identifiaction of bacteriocin ISK-1, a new lantibiotic produced by Pediococcus sp. ISK-1.", Biosci. Biotechnol. Biochem. 62:2341-2345(1998).
NUCLEOTIDE SEQUENCE.
Sashihara T., Kimura H., Higuchi T., Matsusaki H., Sonomoto K., Ishizaki A.
"", Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
PubMed=15322349;DOI=10.1271/bbb.68.1663
Aso Y., Sashihara T., Nagao J., Kanemasa Y., Koga H., Hashimoto T., Higuchi T., Adachi A., Nomiyama H., Ishizaki A., Nakayama J., Sonomoto K.
"Characterization of a Gene Cluster of Staphylococcus warneri ISK-1 Encoding the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", Biosci. Biotechnol. Biochem. 68:1663-1671(2004).
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
MEDLINE=21036904;PubMed=11193411;DOI=10.1271/bbb.64.2420
Sashihara T., Kimura H., Higuchi T., Adachi A., Matsusaki H., Sonomoto K., Ishizaki A.
"A Novel Lantibiotic, Nukacin ISK-1, of Staphylococcus warneri ISK-1: Cloning of the Structural Gene and Identification of the Structure.", Biosci. Biotechnol. Biochem. 64:2420-2428(2000).
NUCLEOTIDE SEQUENCE.
PubMed=16233732;
Aso Y., Nagao J., Koga H., Okuda K., Kanemasa Y., Sashihara T., Nakayama J., Sonomoto K.
"Heterologous Expression and Functional Analysis of the Gene Cluster for Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1.", J. Biosci. Bioeng. 98:429-436(2004).
NUCLEOTIDE SEQUENCE, STRAIN=ISK-1; PLASMID=pPI-1
PubMed=15737403;DOI=10.1016/j.plasmid.2004.08.003
Aso Y., Koga H., Sashihara T., Nagao J., Kanemasa Y., Nakayama J., Sonomoto K.
"Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1.", Plasmid 53:164-178(2005).
FUNCTION AND MECHANISM OF ACTION
PubMed=25229869;DOI=10.1080/09168451.2014.918485
Roy U., Islam M.R., Nagao J., Iida H., Mahin A., Li M., Zendo T., Nakayama J., Sonomoto K.
"Bactericidal activity of nukacin ISK-1: an alternative mode of action.", Biosci Biotechnol Biochem 2014, 78 (7):1270-3.
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Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 KKKSGVIPTV SHDCHMNSFQ FVFTCCS

Wheel representation
wheel representation of Nukacin ISK-1
3D Structure
Protein sequence annotations
Features of Nukacin ISK-1

T
FeaturePosition(s)LengthDescription
PEPTIDE1↔2727
Lantibiotic nukacin.
Feature identifier = PRO_0000307095.
Modified residue242,3-didehydrobutyrine (Probable).
Cross-link9↔146Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link11↔2515Lanthionine (Ser-Cys) (Probable).
Cross-link18↔269Lanthionine (Ser-Cys) (Probable).
Composition
Hydrophobicity

Composition
Formula C132 H204 N36 O38 S4
Absent amino acids AELRWY
Common amino acids S
Mass (Da) 3049.96
Net charge +4
Isoelectric point 8.53
Basic residues 5
Acidic residues 1
Hydrophobic residues 7
Polar residues 11
Aliphatic residues 4
Tiny residues 5
Boman Index -32.5
Hydropathy Index -0.01
Aliphatic Index 46.67
Instability Index 17.84 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 125 M-1 cm-1
Absorbance 280nm 4.81

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