Bacteriocin: streptococcin A-FF22
Accession: BAC030
Classification
Class: Lantibiotic
Genetics
Gene: scnA
Producer and target organisms
Producer Organism: Streptococcus pyogenes [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsactive on certain Gram-positive bacteria
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. extracellular form.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. extracellular form.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: P36501
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=FF22
MEDLINE=93319301;PubMed=8328813;
Hynes W.L., Ferretti J.J., Tagg J.R.
"Cloning of the gene encoding Streptococcin A-FF22, a novel lantibiotic produced by Streptococcus pyogenes, and determination of its nucleotide sequence.", Appl. Environ. Microbiol. 59:1969-1971(1993).
MEDLINE=93319301;PubMed=8328813;
Hynes W.L., Ferretti J.J., Tagg J.R.
"Cloning of the gene encoding Streptococcin A-FF22, a novel lantibiotic produced by Streptococcus pyogenes, and determination of its nucleotide sequence.", Appl. Environ. Microbiol. 59:1969-1971(1993).
[Ref. 2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=FF22
McLaughlin R.E., Hynes W.L.
"", Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
McLaughlin R.E., Hynes W.L.
"", Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
PROTEIN SEQUENCE OF 26-51, STRAIN=FF22
MEDLINE=94170794;PubMed=8125103;
Jack R.W., Carne A., Metzger J., Stefanovic S., Sahl H.-G., Jung G., Tagg J.R.
"Elucidation of the structure of SA-FF22, a lanthionine-containing antibacterial peptide produced by Streptococcus pyogenes strain FF22.", Eur. J. Biochem. 220:455-462(1994).
MEDLINE=94170794;PubMed=8125103;
Jack R.W., Carne A., Metzger J., Stefanovic S., Sahl H.-G., Jung G., Tagg J.R.
"Elucidation of the structure of SA-FF22, a lanthionine-containing antibacterial peptide produced by Streptococcus pyogenes strain FF22.", Eur. J. Biochem. 220:455-462(1994).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene106 | scnK | ScnK | complement(244..1605) |
| BACGene107 | scnR | ScnR | complement(1623..2321) |
| BACGene108 | scnA | streptococcin A-FF22 precursor antibacterial lantibiotic | 2519..2674 |
| BACGene109 | scnA1 | ScnA1 | 2874..3029 |
| BACGene110 | scnM | ScnM | 3050..5833 |
| BACGene111 | scnT | ScnT | 5856..7925 |
| BACGene112 | scnF | ScnF | 8037..8936 |
| BACGene113 | scnE | ScnE | 8948..9712 |
| BACGene114 | scnG | ScnG | 9728..10441 |
| BACGene115 | tnpA | TnpA | complement(10555..11650) |
Protein sequence
| ........10 ........20 ........30 | | | GKNGVFKTIS HECHLNTWAF LATCCS |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔26 | 26 | Lantibiotic streptococcin A-FF22. Feature identifier = PRO_0000017135. |
| Modified residue | 23 | 2,3-didehydrobutyrine (By similarity). | |
| Cross-link | 8↔13 | 6 | Beta-methyllanthionine (Thr-Cys) (Bysimilarity). |
| Cross-link | 10↔24 | 15 | Lanthionine (Ser-Cys) (By similarity). |
| Cross-link | 17↔25 | 9 | Beta-methyllanthionine (Thr-Cys) (Bysimilarity). |
Composition
Hydrophobicity
Composition
| Formula | C126
H191
N35
O36
S3 |
| Absent amino acids | DMPQRY |
| Common amino acids | CT |
| Mass (Da) | 2886.73 |
| Net charge | +3 |
| Isoelectric point | 8.07 |
| Basic residues | 4 |
| Acidic residues | 1 |
| Hydrophobic residues | 9 |
| Polar residues | 12 |
| Aliphatic residues | 4 |
| Tiny residues | 6 |
| Boman Index | -18.59 |
| Hydropathy Index | 0.11 |
| Aliphatic Index | 63.85 |
| Instability Index | 9.4 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 5625 M-1 cm-1 |
| Absorbance 280nm | 225 |
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