Bacteriocin: mutacin H-29B

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Bacteriocin: mutacin H-29B

Accession: BAC025

Classification
Class: Lantibiotic
Genetics
Gene: Unidentified
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic) Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: P84110

PDB EntryUnknown

PROTEIN SEQUENCE, FUNCTION, AND POST-TRANSLATIONAL MODIFICATIONS, STRAIN=29B
Nicolas G.
"Studies of mutacin H-29B.", Thesis (2004), Universite Laval, Canada.
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Gene structure
genome of mutacin H-29B

Gene idNameDescriptionLocation
BACGene82mutAmutacin II lantibiotic496..657
BACGene83mutMorf1726..3422
mutAmutM
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 NRWWQGVVPT VSYECRMNSW QHVFTCC

Wheel representation
wheel representation of mutacin H-29B
Protein sequence annotations
Features of mutacin H-29B

T
FeaturePosition(s)LengthDescription
PEPTIDE1↔2727
Lantibiotic mutacin-2.
Feature identifier = PRO_0000017129.
Modified residue252,3-didehydrobutyrine.
Cross-link10↔156Beta-methyllanthionine (Thr-Cys).
Cross-link12↔2615Lanthionine (Ser-Cys).
Cross-link19↔279Lanthionine (Ser-Cys).
MUTAGEN1Missing: No protein is secreted.
MUTAGEN7V->A: No loss of activity.
MUTAGEN9P->A: Low activity, less than 10% ofwild-type.
MUTAGEN10T->A: Loss of secretion.
MUTAGEN10T->S: No loss of activity.
MUTAGEN15C->A: Loss of activity.
MUTAGEN26C->A: Loss of activity.
MUTAGEN27C->A: Low activity, less than 10% ofwild-type level.
Composition
Hydrophobicity

Composition
Formula C146 H202 N40 O36 S2
Absent amino acids ADIKL
Common amino acids V
Mass (Da) 3
Net charge +2
Isoelectric point 8.06
Basic residues 3
Acidic residues 1
Hydrophobic residues 8
Polar residues 11
Aliphatic residues 4
Tiny residues 3
Boman Index -44.49
Hydropathy Index -0.359
Aliphatic Index 42.96
Instability Index 51.46 (unstable)
Half Life Mammalian : 1.4 hour
Yeast : 3 min
E. coli : >10 hour
Extinction Coefficient 18115 M-1 cm-1
Absorbance 280nm 696.73

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