Bacteriocin: mutacin H-29B
Accession: BAC025
Classification
Class: Lantibiotic
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Streptococcus mutans [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsGram-positive bacteria: Micrococcus luteus - Staphylococcus aureus - Streptococcus - Peptostreptococcus micros - Pediococcus acidilactici - Clostridium sporogenes - Corynebacterium diphtheriae - Actinomyces viscosus - Gardnerella vaginalis - Propionibacterium acnes - Listeria monocytogenes - Mycobacterium smegmatis -
Gram-negative bacteria: Campylobacter jejuni - Helicobacter pylori - Nesseria gonorrhoeae
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic) Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic) Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: P84110
PDB EntryUnknown
[Ref. 1]
PROTEIN SEQUENCE, FUNCTION, AND POST-TRANSLATIONAL MODIFICATIONS, STRAIN=29B
Nicolas G.
"Studies of mutacin H-29B.", Thesis (2004), Universite Laval, Canada.
Nicolas G.
"Studies of mutacin H-29B.", Thesis (2004), Universite Laval, Canada.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene82 | mutA | mutacin II lantibiotic | 496..657 |
| BACGene83 | mutM | orf1 | 726..3422 |
Protein sequence
| ........10 ........20 ........30 | | | NRWWQGVVPT VSYECRMNSW QHVFTCC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔27 | 27 | Lantibiotic mutacin-2. Feature identifier = PRO_0000017129.
|
| Modified residue | 25 | 2,3-didehydrobutyrine. | |
| Cross-link | 10↔15 | 6 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 12↔26 | 15 | Lanthionine (Ser-Cys). |
| Cross-link | 19↔27 | 9 | Lanthionine (Ser-Cys). |
| MUTAGEN | 1 | Missing: No protein is secreted. | |
| MUTAGEN | 7 | V->A: No loss of activity. | |
| MUTAGEN | 9 | P->A: Low activity, less than 10% ofwild-type. | |
| MUTAGEN | 10 | T->A: Loss of secretion. | |
| MUTAGEN | 10 | T->S: No loss of activity. | |
| MUTAGEN | 15 | C->A: Loss of activity. | |
| MUTAGEN | 26 | C->A: Loss of activity. | |
| MUTAGEN | 27 | C->A: Low activity, less than 10% ofwild-type level. |
Composition
Hydrophobicity
Composition
| Formula | C146
H202
N40
O36
S2 |
| Absent amino acids | ADIKL |
| Common amino acids | V |
| Mass (Da) | 3 |
| Net charge | +2 |
| Isoelectric point | 8.06 |
| Basic residues | 3 |
| Acidic residues | 1 |
| Hydrophobic residues | 8 |
| Polar residues | 11 |
| Aliphatic residues | 4 |
| Tiny residues | 3 |
| Boman Index | -44.49 |
| Hydropathy Index | -0.359 |
| Aliphatic Index | 42.96 |
| Instability Index | 51.46 (unstable) |
| Half Life |
Mammalian : 1.4 hour Yeast : 3 min E. coli : >10 hour |
| Extinction Coefficient | 18115 M-1 cm-1 |
| Absorbance 280nm | 696.73 |
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