Bacteriocin: Ruminococcin-A
Accession: BAC023
Classification
Class: Lantibiotic
Genetics
Gene: rumA3
Producer and target organisms
Producer Organism: Ruminococcus gnavus [Gram-positive]
Taxonomy: Bacteria
FirmicutesClostridiaClostridialesLachnospiraceaeRuminococcus
Target organismsactive on Gram-positive bacteria: pathogenic clostridia - Bifidobacterium longum
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. RANGE=24-47; NOTE=Ref.1.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. RANGE=24-47; NOTE=Ref.1.
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), PROTEIN SEQUENCE OF 24-44, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY, STRAIN=E1
MEDLINE=21417035;PubMed=11526013;DOI=10.1128/AEM.67.9.4111-4118.2001
Dabard J., Bridonneau C., Phillipe C., Anglade P., Molle D., Nardi M., Ladire M., Girardin H., Marcille F., Gomez A., Fons M.
"Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.", Appl. Environ. Microbiol. 67:4111-4118(2001).
MEDLINE=21417035;PubMed=11526013;DOI=10.1128/AEM.67.9.4111-4118.2001
Dabard J., Bridonneau C., Phillipe C., Anglade P., Molle D., Nardi M., Ladire M., Girardin H., Marcille F., Gomez A., Fons M.
"Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.", Appl. Environ. Microbiol. 67:4111-4118(2001).
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), STRAIN=LEMB04, LEMB53, LEMV58, LEMV66, LEMV95, and LEMV99
MEDLINE=22083478;PubMed=12089024;DOI=10.1128/AEM.68.7.3424-3431.2002
Marcille F., Gomez A., Joubert P., Ladire M., Veau G., Clara A., Gavini F., Willems A., Fons M.
"Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.", Appl. Environ. Microbiol. 68:3424-3431(2002).
MEDLINE=22083478;PubMed=12089024;DOI=10.1128/AEM.68.7.3424-3431.2002
Marcille F., Gomez A., Joubert P., Ladire M., Veau G., Clara A., Gavini F., Willems A., Fons M.
"Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.", Appl. Environ. Microbiol. 68:3424-3431(2002).
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), DEVELOPMENTAL STAGE, AND INDUCTION, STRAIN=E1
MEDLINE=21617361;PubMed=11741840;DOI=10.1128/JB.184.1.18-28.2002
Gomez A., Ladire M., Marcille F., Fons M.
"Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.", J. Bacteriol. 184:18-28(2002).
MEDLINE=21617361;PubMed=11741840;DOI=10.1128/JB.184.1.18-28.2002
Gomez A., Ladire M., Marcille F., Fons M.
"Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.", J. Bacteriol. 184:18-28(2002).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene68 | hypothetical protein ORF1 | 1..297 | |
| BACGene69 | rumR2 | RumR2 protein putative response-regulator | complement(561..911) |
| BACGene70 | rumH | RumH protein putative ABC-transporter integral membrane protein immunity to ruminococcin A | complement(958..1254) |
| BACGene71 | rumG | RumG protein putative ABC-transporter integral membrane protein immunity to ruminococcin A | complement(1261..2025) |
| BACGene72 | rumE | RumE protein putative ABC-transporter integral membrane protein immunity to ruminococcin A | complement(2096..2764) |
| BACGene73 | rumF | RumF protein putative ABC-transporter (ATP-binding domain) immunity to ruminococcin A | complement(2761..3525) |
| BACGene74 | rumR | RumR protein putative response-regulator | 3674..4375 |
| BACGene75 | rumK | RumK protein putative histidine-kinase | 4380..5759 |
| BACGene76 | rumA1 | pre-RumA ruminococcin A pre-lantibiotic | 5926..6069 |
| BACGene77 | rumA2 | pre-RumA ruminococcin A pre-lantibiotic | 6087..6230 |
| BACGene78 | rumA3 | pre-RumA ruminococcin A pre-lantibiotic | 6249..6392 |
| BACGene79 | rumM | RumM protein putative ruminococcin A modifying enzyme | 6551..9295 |
| BACGene80 | rumT | RumT protein putative ABC-transporter | 9314..11380 |
| BACGene81 | rumT | hypothetical protein ORF14 | 11898..12578 |
Protein sequence
| ........10 ........20 ........30 | | | GNGVLKTISH ECNMNTWQFL FTCC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔24 | 24 | Ruminococcin-A. Feature identifier = PRO_0000017127. |
| Modified residue | 16 | 2,3-didehydrobutyrine. | |
| Cross-link | 7↔12 | 6 | Beta-methyllanthionine (Thr-Cys) (Bysimilarity). |
| Cross-link | 9↔23 | 15 | Lanthionine (Ser-Cys) (Probable). |
| Cross-link | 22↔24 | 3 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Composition
Hydrophobicity
Composition
| Formula | C119
H180
N32
O35
S4 |
| Absent amino acids | ADPRY |
| Common amino acids | CNT |
| Mass (Da) | 2765.57 |
| Net charge | +1 |
| Isoelectric point | 7.18 |
| Basic residues | 2 |
| Acidic residues | 1 |
| Hydrophobic residues | 7 |
| Polar residues | 12 |
| Aliphatic residues | 4 |
| Tiny residues | 3 |
| Boman Index | -18.43 |
| Hydropathy Index | 0.09 |
| Aliphatic Index | 60.83 |
| Instability Index | 1.61 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 5625 M-1 cm-1 |
| Absorbance 280nm | 244.57 |
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