Bacteriocin: Ruminococcin-A

Default view

Bacteriocin: Ruminococcin-A

Accession: BAC023

Classification
Class: Lantibiotic
Genetics
Gene: rumA3
Producer and target organisms

Producer Organism: Ruminococcus gnavus [Gram-positive]

Taxonomy: BacteriaFirmicutesClostridiaClostridialesLachnospiraceaeRuminococcus

Target organismsactive on Gram-positive bacteria: pathogenic clostridia - Bifidobacterium longum

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. RANGE=24-47; NOTE=Ref.1.
Uniprot and PDB links

UniProt Entry: P83674 P83676 P83677 Q8VLK0 Q9K381

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), PROTEIN SEQUENCE OF 24-44, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY, STRAIN=E1
MEDLINE=21417035;PubMed=11526013;DOI=10.1128/AEM.67.9.4111-4118.2001
Dabard J., Bridonneau C., Phillipe C., Anglade P., Molle D., Nardi M., Ladire M., Girardin H., Marcille F., Gomez A., Fons M.
"Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.", Appl. Environ. Microbiol. 67:4111-4118(2001).
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), STRAIN=LEMB04, LEMB53, LEMV58, LEMV66, LEMV95, and LEMV99
MEDLINE=22083478;PubMed=12089024;DOI=10.1128/AEM.68.7.3424-3431.2002
Marcille F., Gomez A., Joubert P., Ladire M., Veau G., Clara A., Gavini F., Willems A., Fons M.
"Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.", Appl. Environ. Microbiol. 68:3424-3431(2002).
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), DEVELOPMENTAL STAGE, AND INDUCTION, STRAIN=E1
MEDLINE=21617361;PubMed=11741840;DOI=10.1128/JB.184.1.18-28.2002
Gomez A., Ladire M., Marcille F., Fons M.
"Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.", J. Bacteriol. 184:18-28(2002).
Loading...
End Note
Reference Manager
BibTeX
Gene structure
genome of Ruminococcin-A

Gene idNameDescriptionLocation
BACGene68hypothetical protein ORF11..297
BACGene69rumR2RumR2 protein putative response-regulatorcomplement(561..911)
BACGene70rumHRumH protein putative ABC-transporter integral membrane protein immunity to ruminococcin Acomplement(958..1254)
BACGene71rumGRumG protein putative ABC-transporter integral membrane protein immunity to ruminococcin Acomplement(1261..2025)
BACGene72rumERumE protein putative ABC-transporter integral membrane protein immunity to ruminococcin Acomplement(2096..2764)
BACGene73rumFRumF protein putative ABC-transporter (ATP-binding domain) immunity to ruminococcin Acomplement(2761..3525)
BACGene74rumRRumR protein putative response-regulator3674..4375
BACGene75rumKRumK protein putative histidine-kinase4380..5759
BACGene76rumA1pre-RumA ruminococcin A pre-lantibiotic5926..6069
BACGene77rumA2pre-RumA ruminococcin A pre-lantibiotic6087..6230
BACGene78rumA3pre-RumA ruminococcin A pre-lantibiotic6249..6392
BACGene79rumMRumM protein putative ruminococcin A modifying enzyme6551..9295
BACGene80rumTRumT protein putative ABC-transporter9314..11380
BACGene81rumThypothetical protein ORF1411898..12578
rumR2rumHrumGrumErumFrumRrumKrumA1rumA2rumA3rumMrumTrumT
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 GNGVLKTISH ECNMNTWQFL FTCC

Wheel representation
wheel representation of Ruminococcin-A
Protein sequence annotations
Features of Ruminococcin-A

T
FeaturePosition(s)LengthDescription
PEPTIDE1↔2424
Ruminococcin-A.
Feature identifier = PRO_0000017127.
Modified residue162,3-didehydrobutyrine.
Cross-link7↔126Beta-methyllanthionine (Thr-Cys) (Bysimilarity).
Cross-link9↔2315Lanthionine (Ser-Cys) (Probable).
Cross-link22↔243Beta-methyllanthionine (Thr-Cys)(Probable).
Composition
Hydrophobicity

Composition
Formula C119 H180 N32 O35 S4
Absent amino acids ADPRY
Common amino acids CNT
Mass (Da) 2765.57
Net charge +1
Isoelectric point 7.18
Basic residues 2
Acidic residues 1
Hydrophobic residues 7
Polar residues 12
Aliphatic residues 4
Tiny residues 3
Boman Index -18.43
Hydropathy Index 0.09
Aliphatic Index 60.83
Instability Index 1.61 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 5625 M-1 cm-1
Absorbance 280nm 244.57

Users comments for Ruminococcin-A

No comments found

Login or register to submit a comment for Ruminococcin-A.