Bacteriocin: streptin

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Bacteriocin: streptin

Accession: BAC021

Classification
Class: lantibiotic
Genetics
Gene: srtA
Producer and target organisms

Producer Organism: Streptococcus pyogenes [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsactive on certain Gram-positive bacteria

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor (By similarity). acids at the N-terminus) is probably due to incomplete processing. Other forms also exist, some of which are due to differing levels of dehydration.
Uniprot and PDB links

UniProt Entry: P0C0H8 Q9FDV1

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=BL-T
MEDLINE=21226666;PubMed=11328600;
Karaya K., Shimizu T., Taketo A.
"New gene cluster for lantibiotic streptin possibly involved in streptolysin S formation.", J. Biochem. 129:769-775(2001).
PROTEIN SEQUENCE OF 21-29, AND CHARACTERIZATION OF ANTIBIOTIC ACTIVITY, STRAIN=M25
MEDLINE=22617465;PubMed=12732544;DOI=10.1128/AEM.69.5.2737-2747.2003
Wescombe P.A., Tagg J.R.
"Purification and characterization of streptin, a type A1 lantibiotic produced by Streptococcus pyogenes.", Appl. Environ. Microbiol. 69:2737-2747(2003).
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End Note
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BibTeX
Gene structure
genome of streptin

Gene idNameDescriptionLocation
BACGene52srtISrtI253..570
BACGene53srtRSrtR721..1407
BACGene54srtKSrtK1400..2746
BACGene55srtASrtA latibiotics precursor2903..3043
BACGene56srtTSrtT3158..4948
BACGene57srtCSrtC4932..6176
BACGene58srtBSrtB6195..9161
BACGene59srtFSrtF9167..9856
BACGene60srtESrtE9862..10611
BACGene61srtGSrtG10614..11336
srtIsrtRsrtKsrtAsrtTsrtCsrtBsrtFsrtEsrtG
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 GSRYLCTPGS CWKLVCFTTT VK

Wheel representation
wheel representation of streptin
Protein sequence annotations
Features of streptin


FeaturePosition(s)LengthDescription
PEPTIDE1↔2222
Lantibiotic streptin.
Feature identifier = PRO_0000017139.
Composition
Hydrophobicity

Composition
Formula C109 H172 N28 O30 S3
Absent amino acids ADEHIMNQ
Common amino acids T
Mass (Da) 2469.26
Net charge +3
Isoelectric point 8.95
Basic residues 3
Acidic residues 0
Hydrophobic residues 6
Polar residues 12
Aliphatic residues 4
Tiny residues 4
Boman Index -14.29
Hydropathy Index 0.23
Aliphatic Index 61.82
Instability Index 50.1 (unstable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 7115 M-1 cm-1
Absorbance 280nm 338.81

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