Bacteriocin: mutacin 1140 (Mutacin III)

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Bacteriocin: mutacin 1140 (Mutacin III)

Accession: BAC020

Classification
Class: Lantibiotic
Genetics
Gene: lanA; Synonyms=mutA
Producer and target organisms

Producer Organism: Streptococcus mutans [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsactive on Gram-positive bacteria

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: O68586

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JH1005
MEDLINE=98261521;PubMed=9596742;
Hillman J.D., Novak J., Sagura E., Gutierrez J.A., Brooks T.A., Crowley P.J., Hess M., Azizi A., Leung K.-P., Cvitkovitch D., Bleiweis A.S.
"Genetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans.", Infect. Immun. 66:2743-2749(1998).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=UA787
MEDLINE=99402724;PubMed=10473390;
Qi F., Chen P., Caufield P.W.
"Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes.", Appl. Environ. Microbiol. 65:3880-3887(1999).
PROTEIN SEQUENCE OF 42-63, MASS SPECTROMETRY, AND STRUCTURE BY NMR.
MEDLINE=20534754;PubMed=11082191;
Smith L., Novak J., Rocca J., McClung S., Hillman J.D., Edison A.S.
"Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics.", Eur. J. Biochem. 267:6810-6816(2000).
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Gene structure
genome of mutacin 1140 (Mutacin III)

Gene idNameDescriptionLocation
BACGene44mutRMutR transcription regulator; similar to Streptococcus gordonii Rgg and Streptococcusmutans mutacin II operon encoded MutR205..1059
BACGene45mutAMutA precursor prepropeptide of mutacin III; identical to mutacin 1140; similar to mutacinNY266, epidermin and gallidermin1646..1837
BACGene46mutA'MutA' similar to MutA; not required for mutacin III production.1904..2098
BACGene47mutBMutB modifying enzyme for promutacin III; similar to SpaB, NisB and EpiB2253..5225
BACGene48mutCMutC modifying enzyme for promutacin III; similar to EpiC, SpaC, NisC, EciC and PepC5212..6486
BACGene49mutDMutD may function in C-terminal oxadative decarboxylation of promutacin III modifying enzyme for promutacin III; similar to EpiD and other flavoproteins6509..7075
BACGene50mutPMutP processing of premutacin III serine protease; similar to NisP, EpiP and serine proteases7080..8423
BACGene51mutTMutT transportation of premutacin III ABC transporter; similar to LmrA, PepT and ABC transporters8424..10049
mutRmutAmutA'mutBmutCmutDmutPmutT
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 FKSWSLCTPG CARTGSFNSY CC

Wheel representation
wheel representation of mutacin 1140 (Mutacin III)
Protein sequence annotations
Features of mutacin 1140 (Mutacin III)

ST
FeaturePosition(s)LengthDescription
PEPTIDE1↔2222
Lantibiotic mutacin-1140.
Feature identifier = PRO_0000017131.
Modified residue52,3-didehydroalanine (Ser).
Modified residue142,3-didehydrobutyrine.
Cross-link3↔75Lanthionine (Ser-Cys).
Cross-link8↔114Beta-methyllanthionine (Thr-Cys).
Cross-link16↔216Lanthionine (Ser-Cys).
Cross-link19↔224S-(2-aminovinyl)-D-cysteine (Ser-Cys).
Composition
Hydrophobicity

Composition
Formula C104 H152 N28 O31 S4
Absent amino acids DEHIMQV
Common amino acids CS
Mass (Da) 2437.1
Net charge +2
Isoelectric point 8.34
Basic residues 2
Acidic residues 0
Hydrophobic residues 5
Polar residues 14
Aliphatic residues 1
Tiny residues 7
Boman Index -23.97
Hydropathy Index 0
Aliphatic Index 22.27
Instability Index 13.67 (stable)
Half Life Mammalian : 1.1 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 7240 M-1 cm-1
Absorbance 280nm 344.76

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