Bacteriocin: salivaricin-A sa
Accession: BAC016
Classification
Class: Lantibiotic
Genetics
Gene: salA
Producer and target organisms
Producer Organism: Streptococcus salivarius [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsactive on Gram-positive bacteria
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: P36500
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 27-42, STRAIN=20P3
MEDLINE=93362982;PubMed=8357242;
Ross K.F., Ronson C.W., Tagg J.R.
"Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3.", Appl. Environ. Microbiol. 59:2014-2021(1993).
MEDLINE=93362982;PubMed=8357242;
Ross K.F., Ronson C.W., Tagg J.R.
"Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3.", Appl. Environ. Microbiol. 59:2014-2021(1993).
[Ref. 2]
SEQUENCE REVISION TO N-TERMINUS, STRAIN=20P3
Upton M., Tagg J.R., Jenkinson H.F.
"Salivaricin A gene cluster from Streptococcus salivarius.", Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
Upton M., Tagg J.R., Jenkinson H.F.
"Salivaricin A gene cluster from Streptococcus salivarius.", Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene22 | salA | SalA lanbiotic salivaricin A prepropeptide | 589..735 |
| BACGene23 | salB | SalB putative salivaricin A modification enzyme; amino acid dehydration | 820..2466 |
| BACGene24 | salC | SalC putative salivaricin A modification enzyme; thioether bridge formation | 2722..3657 |
| BACGene25 | salT | SalT putative salivaricin A ABC transport peptide; contains an N-terminal proteasedomain with motifs characteristic of lantibiotic proteases | 3611..5779 |
| BACGene26 | salX | SalX putative salivaricin A ABC transporter | 5776..6513 |
| BACGene27 | salY | SalY hypothetical protein | 6515..8422 |
| BACGene28 | salK | SalK putative histidine kinase | 8462..10024 |
| BACGene29 | salR | SalR putative response regulator | 10005..10610 |
Protein sequence
| ........10 ........20 ........30 | | | KRGSGWIATI TDDCPNSVFV CC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔22 | 22 | Lantibiotic salivaricin-A. Feature identifier = PRO_0000017133. |
| Cross-link | 9↔14 | 6 | Beta-methyllanthionine (Thr-Cys) (Bysimilarity). |
| Cross-link | 11↔21 | 11 | Beta-methyllanthionine (Thr-Cys) (Bysimilarity). |
| Cross-link | 17↔22 | 6 | Lanthionine (Ser-Cys) (By similarity). |
Composition
Hydrophobicity
Composition
| Formula | C101
H158
N28
O32
S3 |
| Absent amino acids | EHLMQY |
| Common amino acids | CI |
| Mass (Da) | 2391.08 |
| Net charge | 0 |
| Isoelectric point | 6.11 |
| Basic residues | 2 |
| Acidic residues | 2 |
| Hydrophobic residues | 7 |
| Polar residues | 10 |
| Aliphatic residues | 4 |
| Tiny residues | 5 |
| Boman Index | -25.73 |
| Hydropathy Index | 0.2 |
| Aliphatic Index | 66.36 |
| Instability Index | 34.87 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 5625 M-1 cm-1 |
| Absorbance 280nm | 267.86 |
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