Bacteriocin: salivaricin-A sa

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Bacteriocin: salivaricin-A sa

Accession: BAC016

Classification
Class: Lantibiotic
Genetics
Gene: salA
Producer and target organisms
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: P36500

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 27-42, STRAIN=20P3
MEDLINE=93362982;PubMed=8357242;
Ross K.F., Ronson C.W., Tagg J.R.
"Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3.", Appl. Environ. Microbiol. 59:2014-2021(1993).
SEQUENCE REVISION TO N-TERMINUS, STRAIN=20P3
Upton M., Tagg J.R., Jenkinson H.F.
"Salivaricin A gene cluster from Streptococcus salivarius.", Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
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Gene structure
genome of salivaricin-A sa

Gene idNameDescriptionLocation
BACGene22salASalA lanbiotic salivaricin A prepropeptide589..735
BACGene23salBSalB putative salivaricin A modification enzyme; amino acid dehydration820..2466
BACGene24salCSalC putative salivaricin A modification enzyme; thioether bridge formation2722..3657
BACGene25salTSalT putative salivaricin A ABC transport peptide; contains an N-terminal proteasedomain with motifs characteristic of lantibiotic proteases3611..5779
BACGene26salXSalX putative salivaricin A ABC transporter5776..6513
BACGene27salYSalY hypothetical protein6515..8422
BACGene28salKSalK putative histidine kinase8462..10024
BACGene29salRSalR putative response regulator10005..10610
salAsalBsalCsalTsalXsalYsalKsalR
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 KRGSGWIATI TDDCPNSVFV CC

Wheel representation
wheel representation of salivaricin-A sa
Protein sequence annotations
Features of salivaricin-A sa


FeaturePosition(s)LengthDescription
PEPTIDE1↔2222
Lantibiotic salivaricin-A.
Feature identifier = PRO_0000017133.
Cross-link9↔146Beta-methyllanthionine (Thr-Cys) (Bysimilarity).
Cross-link11↔2111Beta-methyllanthionine (Thr-Cys) (Bysimilarity).
Cross-link17↔226Lanthionine (Ser-Cys) (By similarity).
Composition
Hydrophobicity

Composition
Formula C101 H158 N28 O32 S3
Absent amino acids EHLMQY
Common amino acids CI
Mass (Da) 2391.08
Net charge 0
Isoelectric point 6.11
Basic residues 2
Acidic residues 2
Hydrophobic residues 7
Polar residues 10
Aliphatic residues 4
Tiny residues 5
Boman Index -25.73
Hydropathy Index 0.2
Aliphatic Index 66.36
Instability Index 34.87 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 5625 M-1 cm-1
Absorbance 280nm 267.86

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