Bacteriocin: actagardine (Gardimycin)

Default view

Bacteriocin: actagardine (Gardimycin)

Accession: BAC012

Classification
Class: Lantibiotic
Genetics
Gene: Unidentified
Producer and target organisms
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 14-19 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links

UniProt Entry: P56650

PDB Entry1AJ1 resolved by NMR

PRELIMINARY PROTEIN SEQUENCE, AND STRUCTURE BY NMR.
MEDLINE=91008698;PubMed=2211371;
Kettenring J.K., Malabarba A., Vekey K., Cavalleri B.
"Sequence determination of actagardine, a novel lantibiotic, by homonuclear 2D NMR spectroscopy.", J. Antibiot. 43:1082-1088(1990).
PROTEIN SEQUENCE, AND STRUCTURE BY NMR.
MEDLINE=95255286;PubMed=7737178;
Zimmermann N., Metzger J.W., Jung G.
"The tetracyclic lantibiotic actagardine. 1H-NMR and 13C-NMR assignments and revised primary structure.", Eur. J. Biochem. 228:786-797(1995).
STRUCTURE BY NMR.
MEDLINE=97363218;PubMed=9219543;
Zimmermann N., Jung G.
"The three-dimensional solution structure of the lantibiotic murein- biosynthesis-inhibitor actagardine determined by NMR.", Eur. J. Biochem. 246:809-819(1997).
Loading...
End Note
Reference Manager
BibTeX
Protein sequence
 ........10 ........20 
          |          | 
 SSGWVCTLTI ECGTVICAC

Wheel representation
wheel representation of actagardine (Gardimycin)
3D Structure
Protein sequence annotations
Features of actagardine (Gardimycin)


FeaturePosition(s)LengthDescription
PEPTIDE1↔1919
Lantibiotic actagardine.
Feature identifier = PRO_0000043967.
Cross-link1↔66Lanthionine (Ser-Cys).
Cross-link7↔126Beta-methyllanthionine (Thr-Cys).
Cross-link9↔179Beta-methyllanthionine (Thr-Cys).
Cross-link14↔196Beta-methyllanthionine sulfoxide (Thr-Cys).
STRAND7↔1711
Composition
Hydrophobicity

Composition
Formula C81 H132 N20 O27 S4
Absent amino acids DFHKMNPQRY
Common amino acids C
Mass (Da) 1873.4
Net charge -1
Isoelectric point 3.85
Basic residues 0
Acidic residues 1
Hydrophobic residues 7
Polar residues 11
Aliphatic residues 5
Tiny residues 5
Boman Index 12.66
Hydropathy Index 1.27
Aliphatic Index 97.37
Instability Index 103.71 (unstable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 5750 M-1 cm-1
Absorbance 280nm 319.44

Users comments for actagardine (Gardimycin)

No comments found

Login or register to submit a comment for actagardine (Gardimycin).